Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in Europe.

IF 9.5 1区 生物学 Q1 MYCOLOGY
Persoonia Pub Date : 2018-12-01 Epub Date: 2018-02-19 DOI:10.3767/persoonia.2018.40.06
V Guarnaccia, J Z Groenewald, J Woodhall, J Armengol, T Cinelli, A Eichmeier, D Ezra, F Fontaine, D Gramaje, A Gutierrez-Aguirregabiria, J Kaliterna, L Kiss, P Larignon, J Luque, L Mugnai, V Naor, R Raposo, E Sándor, K Z Váczy, P W Crous
{"title":"<i>Diaporthe</i> diversity and pathogenicity revealed from a broad survey of grapevine diseases in Europe.","authors":"V Guarnaccia,&nbsp;J Z Groenewald,&nbsp;J Woodhall,&nbsp;J Armengol,&nbsp;T Cinelli,&nbsp;A Eichmeier,&nbsp;D Ezra,&nbsp;F Fontaine,&nbsp;D Gramaje,&nbsp;A Gutierrez-Aguirregabiria,&nbsp;J Kaliterna,&nbsp;L Kiss,&nbsp;P Larignon,&nbsp;J Luque,&nbsp;L Mugnai,&nbsp;V Naor,&nbsp;R Raposo,&nbsp;E Sándor,&nbsp;K Z Váczy,&nbsp;P W Crous","doi":"10.3767/persoonia.2018.40.06","DOIUrl":null,"url":null,"abstract":"<p><p>Species of <i>Diaporthe</i> are considered important plant pathogens, saprobes, and endophytes on a wide range of plant hosts. Several species are well-known on grapevines, either as agents of pre- or post-harvest infections, including Phomopsis cane and leaf spot, cane bleaching, swelling arm and trunk cankers. In this study we explore the occurrence, diversity and pathogenicity of <i>Diaporthe</i> spp. associated with <i>Vitis vinifera</i> in major grape production areas of Europe and Israel, focusing on nurseries and vineyards. Surveys were conducted in Croatia, Czech Republic, France, Hungary, Israel, Italy, Spain and the UK. A total of 175 <i>Diaporthe</i> strains were isolated from asymptomatic and symptomatic shoots, branches and trunks. A multi-locus phylogeny was established based on five genomic loci (ITS, <i>tef1</i>, <i>cal</i>, <i>his3</i> and <i>tub2</i>), and the morphological characters of the isolates were determined. Preliminary pathogenicity tests were performed on green grapevine shoots with representative isolates. The most commonly isolated species were <i>D. eres</i> and <i>D. ampelina</i>. Four new <i>Diaporthe</i> species described here as <i>D. bohemiae</i>, <i>D. celeris</i>, <i>D. hispaniae</i> and <i>D. hungariae</i> were found associated with affected vines. Pathogenicity tests revealed <i>D. baccae</i>, <i>D. celeris</i>, <i>D. hispaniae</i> and <i>D. hungariae</i> as pathogens of grapevines. No symptoms were caused by <i>D. bohemiae</i>. This study represents the first report of <i>D. ambigua</i> and <i>D. baccae</i> on grapevines in Europe. The present study improves our understanding of the species associated with several disease symptoms on <i>V. vinifera</i> plants, and provides useful information for effective disease management.</p>","PeriodicalId":20014,"journal":{"name":"Persoonia","volume":null,"pages":null},"PeriodicalIF":9.5000,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3767/persoonia.2018.40.06","citationCount":"92","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Persoonia","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3767/persoonia.2018.40.06","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2018/2/19 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 92

Abstract

Species of Diaporthe are considered important plant pathogens, saprobes, and endophytes on a wide range of plant hosts. Several species are well-known on grapevines, either as agents of pre- or post-harvest infections, including Phomopsis cane and leaf spot, cane bleaching, swelling arm and trunk cankers. In this study we explore the occurrence, diversity and pathogenicity of Diaporthe spp. associated with Vitis vinifera in major grape production areas of Europe and Israel, focusing on nurseries and vineyards. Surveys were conducted in Croatia, Czech Republic, France, Hungary, Israel, Italy, Spain and the UK. A total of 175 Diaporthe strains were isolated from asymptomatic and symptomatic shoots, branches and trunks. A multi-locus phylogeny was established based on five genomic loci (ITS, tef1, cal, his3 and tub2), and the morphological characters of the isolates were determined. Preliminary pathogenicity tests were performed on green grapevine shoots with representative isolates. The most commonly isolated species were D. eres and D. ampelina. Four new Diaporthe species described here as D. bohemiae, D. celeris, D. hispaniae and D. hungariae were found associated with affected vines. Pathogenicity tests revealed D. baccae, D. celeris, D. hispaniae and D. hungariae as pathogens of grapevines. No symptoms were caused by D. bohemiae. This study represents the first report of D. ambigua and D. baccae on grapevines in Europe. The present study improves our understanding of the species associated with several disease symptoms on V. vinifera plants, and provides useful information for effective disease management.

Abstract Image

Abstract Image

Abstract Image

从欧洲葡萄病害的广泛调查揭示的多样性和致病性。
该物种被认为是重要的植物病原体、物种和广泛的植物寄主的内生菌。有几个品种在葡萄藤上是众所周知的,作为收获前或收获后感染的媒介,包括甘蔗和叶斑病,甘蔗漂白,肿胀的手臂和树干溃疡病。本研究以葡萄苗圃和葡萄园为研究对象,探讨了与葡萄有关的Diaporthe spp.在欧洲和以色列主要葡萄产区的发生、多样性和致病性。调查在克罗地亚、捷克共和国、法国、匈牙利、以色列、意大利、西班牙和英国进行。从无症状和有症状的芽、枝、干中分离到175株。基于ITS、tef1、cal、his3和tub2 5个基因组位点建立了多位点系统发育模型,并对分离菌株的形态特征进行了分析。对葡萄绿芽进行了初步的致病性试验。最常见的分离种是D. eres和D. ampelina。在受影响的藤蔓上发现了四种新的散斑虫,这里描述为波西米亚散斑虫、celeris散斑虫、西班牙散斑虫和匈牙利散斑虫。致病性试验结果显示,葡萄的病原菌为巴氏弓形虫、celeris弓形虫、西班牙弓形虫和匈牙利弓形虫。波希米亚弧菌未引起任何症状。本研究首次报道了欧洲葡萄藤上的双歧杆菌和双歧杆菌。本研究提高了我们对葡萄属植物几种疾病症状相关物种的认识,并为有效的疾病管理提供了有用的信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Persoonia
Persoonia MYCOLOGY-
CiteScore
17.70
自引率
5.50%
发文量
10
审稿时长
3 months
期刊介绍: Persoonia aspires to publish papers focusing on the molecular systematics and evolution of fungi. Additionally, it seeks to advance fungal taxonomy by employing a polythetic approach to elucidate the genuine phylogeny and relationships within the kingdom Fungi. The journal is dedicated to disseminating high-quality papers that unravel both known and novel fungal taxa at the DNA level. Moreover, it endeavors to provide fresh insights into evolutionary processes and relationships. The scope of papers considered encompasses research articles, along with topical and book reviews.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信