Prevalence of Extended Spectrum Betalactamase (ESBL) and Metallobetalactamase (MBL) Producing Pseudomonas aeruginosa and Acinetobacter baumannii Isolated from Various Clinical Samples.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2018-10-24 eCollection Date: 2018-01-01 DOI:10.1155/2018/6845985

Amandeep Kaur, Satnam Singh

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引用次数: 40

Abstract

This study was conducted with an objective to find the prevalence of extended spectrum betalactamase (ESBL) and metallobetalactamase (MBL) in P. aeruginosa and A. baumannii isolates obtained from various clinical samples. It was conducted in the Department of Microbiology, Adesh Institute of Medical Sciences and Research, Bathinda, over a period of two years from July 2014 to June 2016. Clinical specimens including urine, pus, blood, high vaginal swabs, respiratory samples, and various body fluids were processed and P. aeruginosa and A. baumannii isolates were identified by standard protocols. Antibiotic sensitivity testing for all isolates was done using Kirby-Bauer disc diffusion method. Disc potentiation test was performed to check ESBL and MBL production in these bacteria. Maximum ESBL positive isolates of P. aeruginosa were observed among pus samples and maximum MBL positive isolates were detected in tracheal aspirates. A. baumannii showed maximum positivity for ESBL and MBL production in endotracheal secretions. This study gives an alarming sign towards high prevalence of cephalosporin and carbapenem resistance due to production of extended spectrum betalactamases and metallobetalactamases, respectively. Early detection, stringent antibiotic policies, and compliance towards infection control practices are the best defenses against these organisms.

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广谱β -内酰胺酶(ESBL)和金属β -内酰胺酶(MBL)产生铜绿假单胞菌和鲍曼不动杆菌的临床研究

本研究旨在了解铜绿假单胞菌(P. aeruginosa)和鲍曼假单胞菌(A. baumannii)中延伸谱β -半乳糖酰胺酶(ESBL)和金属β -半乳糖酰胺酶(MBL)的流行情况。该研究于2014年7月至2016年6月在巴欣达Adesh医学科学研究所微生物学系进行，为期两年。临床标本包括尿液、脓液、血液、阴道高拭子、呼吸道样本和各种体液，并根据标准方案鉴定铜绿假单胞菌和鲍曼不动杆菌分离株。采用Kirby-Bauer圆盘扩散法对所有分离株进行抗生素敏感性试验。采用圆盘增强试验检测这些细菌产生ESBL和MBL的情况。脓毒液标本中ESBL阳性菌株最多，气管吸入物中MBL阳性菌株最多。鲍曼不动杆菌在气管分泌物中对ESBL和MBL的产生呈最大阳性。该研究对头孢菌素和碳青霉烯耐药的高发性给出了警告信号，原因分别是产生了广谱β -半乳糖酰胺酶和金属β -半乳糖酰胺酶。早期发现、严格的抗生素政策和遵守感染控制措施是抵御这些微生物的最佳手段。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.