{"title":"[Genotyping Clostridium perfringens strains isolated from patients with symptoms of food poisoning and food samples].","authors":"Klaudia Brodzik, Ewa Augustynowicz","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Clostridium perfringens strains that produce enterotoxin often cause outbreaks of food poisoning or epidemic, therefore is essential to develop and adapt methods useful in epidemiological investigations. The aim of the study was to assess the genetic diversity of C. perfringens strains isolated from patients with symptoms of food poisoning and food samples.</p><p><strong>Methods: </strong>In this study it has been determined the genetic diversity of the pilot group of epidemiologically non- related 39 C. perfringens strains using RAPD (Random Amplified Polymorphic DNA), rep-PCR (Repetitive DNA Element Based PCR) and PFGE (Pulsed Field Gel Electrophoresis) methods.</p><p><strong>Results and conclusions: </strong>It has been obtained comparable results of differentiation of C. perfringens strains isolated from patients with symptoms of food poisoning and food samples using RAOD and rep-PCR methods. It has been also confirmed a wide genetic diversity among C. perfringens strains isolated from different sources epidemiologically non-related using PFGE.</p>","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"68 3-4","pages":"191-201"},"PeriodicalIF":0.0000,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medycyna doswiadczalna i mikrobiologia","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction: Clostridium perfringens strains that produce enterotoxin often cause outbreaks of food poisoning or epidemic, therefore is essential to develop and adapt methods useful in epidemiological investigations. The aim of the study was to assess the genetic diversity of C. perfringens strains isolated from patients with symptoms of food poisoning and food samples.
Methods: In this study it has been determined the genetic diversity of the pilot group of epidemiologically non- related 39 C. perfringens strains using RAPD (Random Amplified Polymorphic DNA), rep-PCR (Repetitive DNA Element Based PCR) and PFGE (Pulsed Field Gel Electrophoresis) methods.
Results and conclusions: It has been obtained comparable results of differentiation of C. perfringens strains isolated from patients with symptoms of food poisoning and food samples using RAOD and rep-PCR methods. It has been also confirmed a wide genetic diversity among C. perfringens strains isolated from different sources epidemiologically non-related using PFGE.
产气荚膜梭菌菌株产生的肠毒素经常引起食物中毒或流行病的爆发，因此开发和调整流行病学调查中有用的方法至关重要。本研究的目的是评估从食物中毒症状患者和食物样本中分离的产气荚膜荚膜杆菌菌株的遗传多样性。方法:采用RAPD (Random Amplified Polymorphic DNA)、rep-PCR (repeated DNA Element Based PCR)和PFGE (Pulsed Field Gel Electrophoresis)等方法，对39株无流行病学相关性的产气荚膜荚膜荚膜菌试验组进行遗传多样性测定。结果与结论:采用RAOD法和rep-PCR法对食物中毒症状患者分离的产气荚膜荚膜原菌与食物样品的鉴别结果相当。从不同来源分离的产气荚膜荚膜杆菌菌株之间也存在广泛的遗传多样性。
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