Apoptotic PET Imaging of Rat Pulmonary Fibrosis With [¹⁸F]ML-8.

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Molecular Imaging Pub Date : 2018-01-01 DOI:10.1177/1536012118795728

Ying Xiong, Dahong Nie, Shaoyu Liu, Hui Ma, Shu Su, Aixia Sun, Jing Zhao, Zhanwen Zhang, Xianhong Xiang, Ganghua Tang

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引用次数: 5

Abstract

Objective: To investigate the value of 2-(3-[¹⁸F]fluoropropyl)-2-methyl-malonic acid ([¹⁸F]ML-8) positron emission tomography (PET) imaging of rat pulmonary fibrosis.

Methods: Male Sprague-Dawley rats were divided into 2 groups, including pulmonary fibrosis model group and control group. The rat model was established by an intratracheal instillation of bleomycin (BLM). Control rats were treated with saline. Positron emission tomography/computed tomography (CT) with [¹⁸F]ML-8 or ¹⁸F-fluorodeoxyglucose ([¹⁸F]FDG) was performed on 2 groups. After PET/CT imaging, lung tissues were collected for histologic examination. Data were analyzed and comparisons between 2 groups were performed using Student t test.

Results: Bleomycin-treated rats showed a higher lung uptake of [¹⁸F]ML-8 than control rats ( P < .05). In BLM-treated rats, the lung to muscle relative uptake ratio of [¹⁸F]ML-8 was also higher than that of [¹⁸F]FDG ( P < .05). Pathological examination showed overproliferation of fibroblasts and deposition of collagen in lungs from BLM-treated rats. Compared to control rats, BLM-treated rats had higher lung hydroxyproline content ( P < .05). Immunofluorescence staining indicated more apoptotic cells in BLM-treated rats than those in control rats. Moreover, the apoptosis rate of lung tissues obtained from BLM-treated rats was higher than that from control rats ( P < .05).

Conclusions: 2-(3-[¹⁸F]fluoropropyl)-2-methyl-malonic acid PET/CT could be used for noninvasive diagnosis of pulmonary fibrosis in a rat model.

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[18F]ML-8对大鼠肺纤维化细胞凋亡的PET显像

目的:探讨2-(3-[18F]氟丙基)-2-甲基丙二酸([18F]ML-8)正电子发射断层扫描(PET)对大鼠肺纤维化的成像价值。方法:雄性Sprague-Dawley大鼠分为肺纤维化模型组和对照组。采用气管内滴注博来霉素(BLM)建立大鼠模型。对照组大鼠给予生理盐水处理。两组分别用[18F]ML-8或18F-氟脱氧葡萄糖([18F]FDG)进行正电子发射断层扫描/计算机断层扫描(CT)。PET/CT成像后，收集肺组织进行组织学检查。采用Student t检验对两组数据进行分析和比较。结果:博莱霉素处理大鼠肺对[18F]ML-8的摄取高于对照组(P < 0.05)。在blm处理的大鼠中，[18F]ML-8的肺肌相对摄取比也高于[18F]FDG (P < 0.05)。病理检查显示blm处理大鼠肺内成纤维细胞过度增生，胶原沉积。与对照组相比，blm处理大鼠肺羟脯氨酸含量较高(P < 0.05)。免疫荧光染色显示，blm处理大鼠的凋亡细胞多于对照组。blm处理大鼠肺组织细胞凋亡率明显高于对照组(P < 0.05)。结论:2-(3-[18F]氟丙基)-2-甲基丙二酸PET/CT可用于大鼠肺纤维化模型的无创诊断。

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来源期刊

Molecular Imaging Biochemistry, Genetics and Molecular Biology-Biotechnology

自引率

3.60%

发文量

期刊介绍： Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.