Effects of Carbon Arc Lamp Irradiation on Wound Healing in a Rat Cutaneous Full-Thickness Wound Model.

Q2 Medicine

Photomedicine and laser surgery Pub Date : 2018-10-18 DOI:10.1089/pho.2018.4447

Jian-Xin Yan, Xuan Liao, Sheng-Hong Li, Hong-Wei Liu, Han-Yu Chang, Nan Dong, Yin-Di Wu, Wen-Li She, Guang-Hui Xie

{"title":"Effects of Carbon Arc Lamp Irradiation on Wound Healing in a Rat Cutaneous Full-Thickness Wound Model.","authors":"Jian-Xin Yan, Xuan Liao, Sheng-Hong Li, Hong-Wei Liu, Han-Yu Chang, Nan Dong, Yin-Di Wu, Wen-Li She, Guang-Hui Xie","doi":"10.1089/pho.2018.4447","DOIUrl":null,"url":null,"abstract":"Objective: The objective of the present study was to investigate the application of a carbon arc lamp on wound healing in a rat cutaneous full-thickness wound model.Background data: In clinical practice, wound healing has been promoted by irradiation with a carbon arc lamp. However, the corresponding mechanism has not been clearly defined.Methods: A cutaneous full-thickness wound on the back of rats was irradiated using a carbon arc lamp at a wavelength peak range of 620-740 nm with 54 J/cm2. Injured sham-irradiated control rats were used as the control. The rats were euthanized after 7, 14, and 21 days, while wound reepithelialization and healing quality were examined by histological analyses with comparison between groups. Cell proliferation was observed by 5-bromo-2'-deoxyuridine (BrdU) immunohistochemical staining.Results: Irradiation by the carbon arc lamp significantly accelerated wound healing. The wound-healing rate in the treated group at day 21 was 98.42% ± 0.56%, compared with 93.58% ± 1.26% in the control group (p < 0.05). Significant increases in the length of epithelial edges, collagen content, and microvessel density were observed in the wound sites in the treated group at days 7, 14, and 21 (p < 0.05). Moreover, the number of BrdU-labeled cells increased in the wound edge at days 7 and 14 due to irradiation (p < 0.05).Conclusions: The results demonstrated that the carbon arc lamp can promote wound healing together with improvement in its quality by stimulating cell proliferation.","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Photomedicine and laser surgery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/pho.2018.4447","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: The objective of the present study was to investigate the application of a carbon arc lamp on wound healing in a rat cutaneous full-thickness wound model.

Background data: In clinical practice, wound healing has been promoted by irradiation with a carbon arc lamp. However, the corresponding mechanism has not been clearly defined.

Methods: A cutaneous full-thickness wound on the back of rats was irradiated using a carbon arc lamp at a wavelength peak range of 620-740 nm with 54 J/cm². Injured sham-irradiated control rats were used as the control. The rats were euthanized after 7, 14, and 21 days, while wound reepithelialization and healing quality were examined by histological analyses with comparison between groups. Cell proliferation was observed by 5-bromo-2'-deoxyuridine (BrdU) immunohistochemical staining.

Results: Irradiation by the carbon arc lamp significantly accelerated wound healing. The wound-healing rate in the treated group at day 21 was 98.42% ± 0.56%, compared with 93.58% ± 1.26% in the control group (p < 0.05). Significant increases in the length of epithelial edges, collagen content, and microvessel density were observed in the wound sites in the treated group at days 7, 14, and 21 (p < 0.05). Moreover, the number of BrdU-labeled cells increased in the wound edge at days 7 and 14 due to irradiation (p < 0.05).

Conclusions: The results demonstrated that the carbon arc lamp can promote wound healing together with improvement in its quality by stimulating cell proliferation.

查看原文本刊更多论文

碳弧灯照射对大鼠皮肤全厚伤口模型愈合的影响

研究目的本研究旨在探讨碳弧灯在大鼠皮肤全厚伤口模型中对伤口愈合的应用：背景资料：在临床实践中，用碳弧灯照射可促进伤口愈合。背景资料：在临床实践中，用碳弧灯照射可促进伤口愈合，但其相应的机制尚未明确：方法：使用波长峰值范围为 620-740 nm、功率为 54 J/cm2 的碳弧灯照射大鼠背部的皮肤全厚伤口。受伤的假照射对照组大鼠作为对照。大鼠分别在 7 天、14 天和 21 天后安乐死，并通过组织学分析和组间比较来检测伤口的再上皮化和愈合质量。通过 5-溴-2'-脱氧尿苷（BrdU）免疫组化染色观察细胞增殖情况：结果：碳弧灯照射能明显加速伤口愈合。第 21 天时，治疗组的伤口愈合率为 98.42% ± 0.56%，而对照组为 93.58% ± 1.26%（p 结论：碳弧灯照射可明显加速伤口愈合：结果表明，碳弧灯可通过刺激细胞增殖促进伤口愈合，同时改善伤口质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Photomedicine and laser surgery 医学-外科

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Photobiomodulation, Photomedicine, and Laser Surgery (formerly Photomedicine and Laser Surgery) is the essential journal for cutting-edge advances and research in phototherapy, low-level laser therapy (LLLT), and laser medicine and surgery. The Journal delivers basic and clinical findings and procedures to improve the knowledge and application of these techniques in medicine.