Effects of social defeat stress on dopamine D2 receptor isoforms and proteins involved in intracellular trafficking.

IF 4.7 2区 心理学 Q1 BEHAVIORAL SCIENCES
Vishwanath Vasudev Prabhu, Thong Ba Nguyen, Yin Cui, Young-Eun Oh, Keon-Hak Lee, Tarique R Bagalkot, Young-Chul Chung
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引用次数: 16

Abstract

Background: Chronic social defeat stress induces depression and anxiety-like behaviors in rodents and also responsible for differentiating defeated animals into stress susceptible and resilient groups. The present study investigated the effects of social defeat stress on a variety of behavioral parameters like social behavior, spatial learning and memory and anxiety like behaviors. Additionally, the levels of various dopaminergic markers, including the long and short form of the D2 receptor, and total and phosphorylated dopamine and cyclic adenosine 3',5'-monophosphate regulated phosphoprotein-32, and proteins involved in intracellular trafficking were assessed in several key brain regions in young adult mice.

Methods: Mouse model of chronic social defeat was established by resident-intruder paradigm, and to evaluate the effect of chronic social defeat, mice were subjected to behavioral tests like spontaneous locomotor activity, elevated plus maze (EPM), social interaction and Morris water maze tests.

Results: Mice were divided into susceptible and unsusceptible groups after 10 days of social defeat stress. The susceptible group exhibited greater decreases in time spent in the open and closed arms compared to the control group on the EPM. In the social interaction test, the susceptible group showed greater increases in submissive and neutral behaviors and greater decreases in social behaviors relative to baseline compared to the control group. Furthermore, increased expression of D2L, D2S, Rab4, and G protein-coupled receptor associated sorting protein-1 was observed in the amygdala of the susceptible group compared to the control group.

Conclusion: These findings suggest that social defeat stress induce anxiety-like and altered social interacting behaviors, and changes in dopaminergic markers and intracellular trafficking-related proteins.

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社会失败应激对参与细胞内运输的多巴胺D2受体亚型和蛋白质的影响。
背景:长期的社会失败应激会诱发啮齿动物的抑郁和焦虑样行为,也是失败动物分化为应激易感性和弹性群体的原因。本研究探讨了社会失败压力对社会行为、空间学习记忆和焦虑行为等行为参数的影响。此外,各种多巴胺能标记物的水平,包括D2受体的长和短形式,总多巴胺和磷酸化多巴胺,环腺苷3',5'-单磷酸调节磷酸化蛋白-32,以及参与细胞内运输的蛋白,在年轻成年小鼠的几个关键大脑区域进行了评估。方法:采用“居民-入侵者”模式建立小鼠慢性社会失败模型,并对小鼠进行自发性运动活动、升高+迷宫(EPM)、社会互动和Morris水迷宫等行为测试,以评价慢性社会失败的影响。结果:小鼠经过10天的社交失败应激后分为易感组和不易感组。与对照组相比,易感组在EPM上花在张开和闭合手臂上的时间明显减少。在社会互动测试中,与对照组相比,易感组相对于基线表现出更大的顺从和中立行为的增加和更大的社会行为的减少。此外,与对照组相比,易感组杏仁核中D2L、D2S、Rab4和G蛋白偶联受体相关分选蛋白-1的表达增加。结论:这些研究结果表明,社会失败应激诱导焦虑样和改变的社会互动行为,以及多巴胺能标记物和细胞内运输相关蛋白的变化。
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来源期刊
Behavioral and Brain Functions
Behavioral and Brain Functions 医学-行为科学
CiteScore
5.90
自引率
0.00%
发文量
11
审稿时长
6-12 weeks
期刊介绍: A well-established journal in the field of behavioral and cognitive neuroscience, Behavioral and Brain Functions welcomes manuscripts which provide insight into the neurobiological mechanisms underlying behavior and brain function, or dysfunction. The journal gives priority to manuscripts that combine both neurobiology and behavior in a non-clinical manner.
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