Bioinspired liver scaffold design criteria.

IF 1.6 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-08-29 DOI:10.1080/15476278.2018.1505137
Giorgio Mattei, Chiara Magliaro, Andrea Pirone, Arti Ahluwalia
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引用次数: 9

Abstract

Maintaining hepatic functional characteristics in-vitro is considered one of the main challenges in engineering liver tissue. As hepatocytes cultured ex-vivo are deprived of their native extracellular matrix (ECM) milieu, developing scaffolds that mimic the biomechanical and physicochemical properties of the native ECM is thought to be a promising approach for successful tissue engineering and regenerative medicine applications. On the basis that the decellularized liver matrix represents the ideal design template for engineering bioinspired hepatic scaffolds, to derive quantitative descriptors of liver ECM architecture, we characterised decellularised liver matrices in terms of their biochemical, viscoelastic and structural features along with porosity, permeability and wettability. Together, these data provide a unique set of quantitative design criteria which can be used to generate guidelines for fabricating biomaterial scaffolds for liver tissue engineering. As proof-of-concept, we investigated hepatic cell response to substrate viscoelasticity. On collagen hydrogels mimicking decellularised liver mechanics, cells showed superior morphology, higher viability and albumin secretion than on stiffer and less viscous substrates. Although scaffold properties are generally inspired by those of native tissues, our results indicate significant differences between the mechano-structural characteristics of untreated and decellularised hepatic tissue. Therefore, we suggest that design rules - such as mechanical properties and swelling behaviour - for engineering biomimetic scaffolds be re-examined through further studies on substrates matching the features of decellularized liver matrices.

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仿生肝支架设计标准。
在体外维持肝功能特征被认为是肝组织工程的主要挑战之一。由于离体培养的肝细胞被剥夺了其天然细胞外基质(ECM)环境,开发模拟天然ECM生物力学和物理化学特性的支架被认为是成功应用组织工程和再生医学的一种很有前途的方法。基于脱细胞肝基质代表了工程化仿生肝支架的理想设计模板,为了推导肝ECM结构的定量描述符,我们对脱细胞肝基体的生物化学、粘弹性和结构特征以及孔隙率、渗透性和润湿性进行了表征。总之,这些数据提供了一套独特的定量设计标准,可用于制定用于肝组织工程的生物材料支架的制造指南。作为概念的证明,我们研究了肝细胞对底物粘弹性的反应。在模拟脱细胞肝脏力学的胶原水凝胶上,细胞比在更硬、粘性更小的基质上表现出更好的形态、更高的活力和白蛋白分泌。尽管支架的特性通常受到天然组织的启发,但我们的研究结果表明,未处理和脱细胞肝组织的机械结构特征之间存在显著差异。因此,我们建议通过对与脱细胞肝基质特征相匹配的基质的进一步研究,重新检验工程仿生支架的设计规则,如机械性能和溶胀行为。
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来源期刊
Organogenesis
Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY
CiteScore
4.10
自引率
4.30%
发文量
6
审稿时长
>12 weeks
期刊介绍: Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.
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