Phylogeny and genetic diversity of the banana Fusarium wilt pathogen Fusarium oxysporum f. sp. cubense in the Indonesian centre of origin

IF 14.1 1区生物学 Q1 MYCOLOGY

Studies in Mycology Pub Date : 2019-03-01 DOI:10.1016/j.simyco.2018.06.003

N. Maryani , L. Lombard , Y.S. Poerba , S. Subandiyah , P.W. Crous , G.H.J. Kema

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引用次数: 147

Abstract

Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt or Panama disease on banana, is one of the major constraints in banana production worldwide. Indonesia is the centre of origin for wild and cultivated bananas, which likely co-evolved with Foc. This study explored the widest possible genetic diversity of Foc by sampling across Indonesia at 34 geographically and environmentally different locations in 15 provinces at six islands. This resulted in a comprehensive collection of ∼200 isolates from 40 different local banana varieties. Isolates were identified and assessed using sequence analysis of the translation elongation factor-1alpha (tef1), the RNA polymerase II largest subunit (rpb1), and the RNA polymerase II second largest subunit (rpb2). Phylogenetic analyses of these genes allowed the identification of 180 isolates of Fusarium oxysporum f. sp. cubense (Foc), and 20 isolates of the Fusarium fujikuroi species complex (FFSC), the Fusarium incarnatum-equiseti species complex (FIESC), and the Fusarium sambucinum species complex (FSSC). Further analyses, incorporating a worldwide collection of Foc strains, revealed nine independent genetic lineages for Foc, and one novel clade in the Fusarium oxysporum species complex (FOSC). Selected isolates from each lineage were tested on the banana varieties Gros Michel and Cavendish to characterise their pathogenicity profiles. More than 65 % of the isolates were diagnosed as Tropical Race 4 (Foc-TR4) due to their pathogenicity to Cavendish banana, which supports the hypothesis that Foc-TR4 is of Indonesian origin. Nine independent genetic lineages for Foc are formally described in this study. This biodiversity has not been studied since the initial description of Foc in 1919. This study provides a detailed overview of the complexity of Fusarium wilt on banana and its diversity and distribution across Indonesia.

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印度尼西亚香蕉枯萎病病原菌Fusarium oxysporum f. sp. cubense的系统发育和遗传多样性

香蕉枯萎病(Fusarium oxysporum f. sp. cubense, Foc)是香蕉枯萎病(Fusarium wilt)或巴拿马病(Panama disease)的致病因子，是制约香蕉生产的主要因素之一。印度尼西亚是野生和栽培香蕉的起源中心，可能与Foc共同进化。本研究通过在印度尼西亚6个岛屿15个省34个地理和环境不同的地点取样，探索了Foc最广泛的遗传多样性。这导致从40个不同的当地香蕉品种中全面收集到约200个分离株。利用翻译延伸因子-1 α (tef1)、RNA聚合酶II最大亚基(rpb1)和RNA聚合酶II第二大亚基(rpb2)的序列分析对分离物进行鉴定和评估。这些基因的系统发育分析鉴定出180株尖孢镰刀菌(Fusarium oxysporum f. sp. cubense, Foc)和20株Fusarium fujikuroi种复合体(FFSC)、Fusarium incarnatum-equiseti种复合体(FIESC)和Fusarium sambucinum种复合体(FSSC)。进一步分析，结合世界范围内收集的Foc菌株，揭示了Foc的9个独立遗传谱系，以及尖孢镰刀菌物种复合体(FOSC)中的一个新分支。从每个谱系中选出的分离株在香蕉品种Gros Michel和Cavendish上进行了测试，以表征它们的致病性谱。由于其对卡文迪什香蕉的致病性，超过65%的分离株被诊断为热带小种4 (focc - tr4)，这支持了focc - tr4起源于印度尼西亚的假设。本研究正式描述了Foc的9个独立遗传谱系。自1919年首次描述Foc以来，这种生物多样性尚未得到研究。本研究提供了香蕉枯萎病的复杂性及其在印度尼西亚的多样性和分布的详细概述。

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来源期刊

Studies in Mycology 生物-真菌学

CiteScore

35.60

自引率

3.00%

发文量

期刊介绍： The international journal Studies in Mycology focuses on advancing the understanding of filamentous fungi, yeasts, and various aspects of mycology. It publishes comprehensive systematic monographs as well as topical issues covering a wide range of subjects including biotechnology, ecology, molecular biology, pathology, and systematics. This Open-Access journal offers unrestricted access to its content. Each issue of Studies in Mycology consists of around 5 to 6 papers, either in the form of monographs or special focused topics. Unlike traditional length restrictions, the journal encourages submissions of manuscripts with a minimum of 50 A4 pages in print. This ensures a thorough exploration and presentation of the research findings, maximizing the depth of the published work.