Taurine enhances mouse cochlear neural stem cells proliferation and differentiation to sprial gangli through activating sonic hedgehog signaling pathway.

IF 1.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Organogenesis Pub Date : 2018-01-01 Epub Date: 2018-08-13 DOI:10.1080/15476278.2018.1477462

Xinghua Huang, Weijing Wu, Peng Hu, Qin Wang

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引用次数: 4

Abstract

To investigate the molecular mechanism underlying taurine-stimulated proliferation and differentiation of cochlear neural stem cells (NSCs) and potential involvement of Sonic Hedgehog (Shh) pathway. The NSCs were characterized with immunofluorescence stained with nestin antibody. Cell viability was determined by MTT assay. The relative proliferation was measured by BrdU incorporation assay. The morphologic index was measured under light microscope. The relative protein level was determined by immunoblotting. Here we presented our findings that taurine stimulated proliferation and neurite outgrowth of NSCs, which was completely abolished by Shh inhibitor cyclopamine. In addition, cyclopamine antagonized taurine's effect on glutamatergic and GABAergic neuron population via suppressing expressions of Ptc-1, Smo and Gli-1. Our data supported the critical role of Shh pathway underlying the protective effect of taurine on auditory neural system.

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牛磺酸通过激活sonic hedgehog信号通路促进小鼠耳蜗神经干细胞向螺旋神经节的增殖和分化。

探讨牛磺酸刺激耳蜗神经干细胞(NSCs)增殖和分化的分子机制以及Sonic Hedgehog (Shh)通路的潜在参与。用nestin抗体免疫荧光染色对NSCs进行表征。MTT法测定细胞活力。用BrdU掺入法测定相对增殖。光镜下测定形态学指标。免疫印迹法测定相对蛋白水平。在这里，我们提出了我们的研究结果，牛磺酸刺激NSCs的增殖和神经突的生长，而Shh抑制剂环巴胺完全消除了这一作用。此外，环巴胺通过抑制Ptc-1、Smo和gli1的表达，拮抗牛磺酸对谷氨酸能和gaba能神经元群体的影响。我们的数据支持Shh通路在牛磺酸对听觉神经系统保护作用中的关键作用。

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来源期刊

Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY

CiteScore

4.10

自引率

4.30%

发文量

审稿时长

>12 weeks

期刊介绍： Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.