{"title":"[The effects of down-regulated ITGB5 expression on the proliferation of keloid fibroblasts].","authors":"Tongtong Yang, Minliang Chen, Kui Ma, Xiaobing Fu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To explore the effects of down-regulated ITGB5 expression on the proliferation of keloid fibroblasts and clarify the possible role of β5-integrin (ITGB5) in keloid.</p><p><strong>Methods: </strong>Construct lentiviral sh-RNA-expression vector targeting ITGB5 and infect keloid fibroblasts, the expression of ITGB5 were detected by Western Blot, the proliferation ability was identified by MTT.</p><p><strong>Results: </strong>The expression quantity of ITGB5 mRNA and protein in KFb group,LV-NC group and LV-KFb group are 1.00 ± 0.00,1.08 ±0.05,0.34 ±0.01 and 0.91 ±0.03,0.93 ±0.02,0.28 ±0.07.Compared with LV-NC group and KFb group, the expression quantity of ITGB5 mRNA and protein in LV-KFb group decreased significantly (P ＜ 0.01).Compared with LV-NC group and KFb group,the proliferation rate decreased significantly in LV-KFb group at 48 h(P ＜ 0.01).</p><p><strong>Conclusions: </strong>These results suggest that ITGB5 can accelerate fibroblasts proliferation in keloids.</p>","PeriodicalId":69147,"journal":{"name":"中华整形外科杂志","volume":" ","pages":"49-52"},"PeriodicalIF":0.0000,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华整形外科杂志","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
Objective: To explore the effects of down-regulated ITGB5 expression on the proliferation of keloid fibroblasts and clarify the possible role of β5-integrin (ITGB5) in keloid.
Methods: Construct lentiviral sh-RNA-expression vector targeting ITGB5 and infect keloid fibroblasts, the expression of ITGB5 were detected by Western Blot, the proliferation ability was identified by MTT.
Results: The expression quantity of ITGB5 mRNA and protein in KFb group,LV-NC group and LV-KFb group are 1.00 ± 0.00,1.08 ±0.05,0.34 ±0.01 and 0.91 ±0.03,0.93 ±0.02,0.28 ±0.07.Compared with LV-NC group and KFb group, the expression quantity of ITGB5 mRNA and protein in LV-KFb group decreased significantly (P ＜ 0.01).Compared with LV-NC group and KFb group,the proliferation rate decreased significantly in LV-KFb group at 48 h(P ＜ 0.01).
Conclusions: These results suggest that ITGB5 can accelerate fibroblasts proliferation in keloids.
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