[Differential expression profile of long non-coding RNA in regressive scar and mature scar].

中华整形外科杂志 Pub Date : 2016-07-01

Jun Li, Qian Li, Yanli Gao, Bei Zhou, Jingyun Li

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引用次数: 0

Abstract

Objective: To analyze the expression profile of long non-coding RNA (lncRNA) in regressive scar and mature scar.

Methods: Hematoxylin-eosin staining and Masson staining were used to examine the histology of regressive scar and mature scar.The technique of IncRNA microarray was used to test the IncRNA expression profile in regressive scar and mature scar. The raw data of lncRNA were pretreated for normalization. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was further performed. Four lncRNA expressions were validated by quantitative PCR.

Results: Compared with mature scar, more fibroblasts and deeper Masson staining color were seen in the regressive scar, a total of 1 275 upregulated more than 3-fold were found in the regressive scar. And 596 down regulated more than seventy percent lncRNA were found in the regressive scar. Pathway analysis indicated that chemokine signaling pathway, TNF signaling pathway, insulin secretion and focal adhesion would play important roles in scar development.

Conclusions: lncRNA expression profiles in the regressive scar is markedly different in comparison with the mature scar,revealing that lncRNA may participate in pathophysiological process of scar maturation.

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[长链非编码RNA在退行性瘢痕和成熟性瘢痕中的差异表达谱]。

目的:分析长链非编码RNA (lncRNA)在退行性瘢痕和成熟性瘢痕中的表达谱。方法:采用苏木精-伊红染色和马松染色对退行性瘢痕和成熟性瘢痕进行组织学观察。采用IncRNA芯片技术检测退行性瘢痕和成熟性瘢痕中IncRNA的表达谱。lncRNA原始数据经预处理归一化处理。进一步进行京都基因与基因组百科全书(KEGG)通路分析。4个lncRNA表达通过定量PCR验证。结果:与成熟瘢痕相比，退行性瘢痕成纤维细胞增多，Masson染色颜色加深，共1 275个表达上调3倍以上。在退行性疤痕中发现了596个超过70%的lncRNA下调。通路分析表明趋化因子信号通路、TNF信号通路、胰岛素分泌和局灶黏附在瘢痕形成中发挥重要作用。结论:lncRNA在退行性瘢痕中的表达谱与成熟性瘢痕的表达谱存在显著差异，提示lncRNA可能参与了瘢痕成熟的病理生理过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

中华整形外科杂志

CiteScore

0.20

自引率

0.00%

发文量

5346