Are We Neglecting Nontuberculous Mycobacteria Just as Laboratory Contaminants? Time to Reevaluate Things.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2018-06-21 eCollection Date: 2018-01-01 DOI:10.1155/2018/8907629

Pooja Sharma, Digvijay Singh, Kusum Sharma, Santwana Verma, Sanjay Mahajan, Anil Kanga

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引用次数: 0

Abstract

Objectives: Nontuberculous mycobacteria (NTM) incidences are on the rise worldwide, including the tuberculosis endemic areas. They should be identified rapidly to the species level and should be carefully differentiated as contamination, colonization, or disease. This study was aimed at determining the prevalence and clinicoepidemiological profile of mycobacteriosis cases.

Materials and methods: Cultures were made on liquid and solid media. NTM were identified by polymerase chain reaction (PCR) restriction analysis (PRA) and gene sequencing. Data was analyzed using Epi-info 7.

Results: Out of the 1042 processed specimens, 16% were positive for M. tuberculosis complex and 1.2% for clinically significant NTM. M. intracellulare was the commonest species isolated. NTM were treated mainly on outdoor basis (92%), involving more extrapulmonary system (62%) and higher age-group of 41-60 years (69%). No significant factor was seen to be associated clinically, radiologically, and biochemically with the NTM infections.

Conclusions: Our study highlights the importance of early diagnosis and differentiation among Mycobacterium tuberculosis and NTM so that these NTM are not underestimated in routine diagnostic procedures merely as environmental or laboratory contaminants.

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我们只是把非结核分枝杆菌当作实验室污染物而忽视了吗？是时候重新评估了。

目的：非结核分枝杆菌（NTM）的发病率在全球范围内呈上升趋势，包括结核病流行地区。应迅速对非结核分枝杆菌进行物种鉴定，并仔细区分其是污染、定植还是疾病。本研究旨在确定分枝杆菌病病例的流行率和临床流行病学特征：在液体和固体培养基上进行培养。通过聚合酶链式反应（PCR）限制分析（PRA）和基因测序鉴定非结核分枝杆菌。使用 Epi-info 7 对数据进行分析：结果：在处理的 1042 份标本中，16% 对复合结核杆菌呈阳性，1.2% 对有临床意义的非结核杆菌呈阳性。细胞内结核杆菌是最常见的分离菌种。前线腺瘤主要在室外治疗（92%），涉及肺外系统较多（62%），41-60 岁年龄组较多（69%）。从临床、放射学和生化角度看，NTM感染与其他因素均无明显关联：我们的研究强调了早期诊断和区分结核分枝杆菌和非结核分枝杆菌的重要性，这样在常规诊断程序中，这些非结核分枝杆菌就不会仅仅作为环境或实验室污染物而被低估。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.