Species A Rotavirus (RVA) Isolated from Sewage in Nigeria, 2014: Close Genetic Relatedness of Partial G, P, and NSP4 Gene Sequences Encoding G1 with Cogent Genes of Other Asian and African Rotaviruses.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2018-06-24 eCollection Date: 2018-01-01 DOI:10.1155/2018/8425621

Babatunde Olanrewaju Motayo, Johnson Adekunle Adeniji, Adedayo Omotayo Faneye

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引用次数: 5

Abstract

Rotavirus has been identified as a major cause of gastroenteritis in Nigeria. There is limited information on the intragenotype diversity of Nigerian rotavirus isolates. We therefore investigated the molecular characteristics of some rotavirus gene sequences detected in sewage from Nigeria. Seven sewage samples, out of a total of 68, tested positive for rotavirus RNA (10.3%). Genotype G1P[4] was the most common genotype (5 isolates) and one isolate for genotypes G1P[8] and G3P[6]. Phylogenetic analysis of the partial VP7 gene of 3 G1P[4] isolates analyzed identified them as genotype G1 Lineage 2 along with Chinese strains with 99.1% to 100% amino acid similarity. Amino acid substitutions D-97→E and S-147→D/N were observed within the 7-1a and 7-2 domains of VP7 gene among the study G1P4 isolates in reference to vaccine strain RotaTeq®. Phylogenetic analysis of the G3P[6] study isolate identified it as genotype G3 Lineage 3, forming a monophyletic cluster with 100% bootstrap value with other West African strains G3 isolates. Phylogenetic analysis of GIP[4] VP4 genes identified them as P4 Lineage 5, while 3 NSP4 gene sequences belonged to genotype E1, while 1 belonged to E2. The results from this study represent phylogenetic analysis of partial gene sequences of environmental group A rotavirus (RVA) isolates from Nigeria.

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2014年从尼日利亚污水中分离的A种轮状病毒(RVA):编码G1的部分G、P和NSP4基因序列与其他亚洲和非洲轮状病毒的强基因具有密切的遗传相关性

轮状病毒已被确定为尼日利亚胃肠炎的主要病因。关于尼日利亚轮状病毒分离株的内型多样性的信息有限。因此，我们研究了在尼日利亚污水中检测到的一些轮状病毒基因序列的分子特征。在68个污水样本中，有7个样本轮状病毒RNA检测呈阳性(10.3%)。基因型G1P[4]是最常见的基因型(5株)，基因型G1P[8]和基因型G3P[6]各有1株。对所分析的3株G1P[4]分离株的VP7部分基因进行系统发育分析，鉴定其与中国菌株的基因型为G1 - Lineage 2，氨基酸相似性为99.1% ~ 100%。与RotaTeq®疫苗株比较，G1P4分离株VP7基因的7-1a和7-2结构域出现了D-97→E和S-147→D/N的氨基酸替换。对G3P[6]研究分离物进行系统发育分析，鉴定其为G3谱系3基因型，与其他西非菌株G3分离物形成一个单系簇，具有100%的自引导值。对GIP[4] VP4基因进行系统发育分析，鉴定为P4谱系5,NSP4基因序列3个属于E1基因型，1个属于E2基因型。本研究结果代表了尼日利亚环境A群轮状病毒(RVA)分离株部分基因序列的系统发育分析。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.