[The experimental study of the effect of ASCs on the skin expansion rate in rabbit].

中华整形外科杂志 Pub Date : 2016-03-01

Zemin Zhang, He Yan, Yongming Yao, Caifeng Wu, Changying Niu, Shengxing Tan, Biaobing Yang

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引用次数: 0

Abstract

Objective: To explore the effect of adipose-derived stem cells (ASCs) on the skin expansion rate in rabbit.

Methods: The rabbit ASCs were isolated from fat tissue and cultured in vitro. The ADSCs were identified by cell immunofluorescence and marked by Edu staining.20 new Zealand rabbits were randomly divided into experimental(n =10) and control group(n =10).An area of 1.5 cm ×1.5 cm on the one side back of each rabbit was tattooed and one 30 ml round expander was implanted subcutaneously. ASCs suspension (1 ml) was injected subcutaneously in the experimental group, while serum free DMEM medium(1 ml) in control group. The expansion was proceeded regularly under constant pressure for 4 weeks.The expanded tattooed square area was measured on the 7th,14th,28th day and analyzed statistically. The expanded skin was harvested for histological study. Immunohistochemical staining was used to detect the expression of vascular endothelial cell marker CD31,and the microvessel density determination. The expression of epidermal growth factor (EGF) and vascular endothelial growth factor(VEGF)was detected by ELISA for skin tissue specificity. Western Blot was used for detection of CK19 in the epidermal cells.

Results: The expanded skin thickness and expansion rate in experimental group were significant higher than those in control group (P ＜ 0.05). Compared with control group, the expression of CK19,CD31 and EGF, VEGF, as well as the microvessel density were all markedly increased in experimental group(P ＜0.05).

Conclusions: ASCs can increase the expansion rate of skin tissue by promotion of angiogenesis and tissue regeneration.

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[ASCs对家兔皮肤扩张率影响的实验研究]。

目的:探讨脂肪源性干细胞(ASCs)对兔皮肤扩张率的影响。方法:从兔脂肪组织中分离培养ASCs。细胞免疫荧光法鉴定ADSCs, Edu染色法标记ADSCs。将20只新西兰兔随机分为试验组(n =10)和对照组(n =10)。在每只兔背部一侧纹1.5 cm ×1.5 cm，皮下植入30 ml圆形扩张器1个。实验组皮下注射ASCs混悬液(1 ml)，对照组皮下注射无血清DMEM培养基(1 ml)。在恒压下定期进行膨胀，持续4周。分别于第7天、第14天、第28天测量扩大后的纹方面积并进行统计分析。取扩张后的皮肤进行组织学研究。免疫组化染色检测血管内皮细胞标志物CD31的表达，微血管密度测定。采用ELISA法检测表皮生长因子(EGF)和血管内皮生长因子(VEGF)的表达，具有皮肤组织特异性。Western Blot法检测表皮细胞中CK19的表达。结果:实验组皮肤扩张厚度和扩张率显著高于对照组(P < 0.05)。与对照组相比，试验组细胞中CK19、CD31、EGF、VEGF的表达及微血管密度均显著升高(P <0.05)。结论:ASCs可通过促进血管生成和组织再生来提高皮肤组织扩张率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

中华整形外科杂志

CiteScore

0.20

自引率

0.00%

发文量

5346