Antimalarial Activity of Croton macrostachyus Stem Bark Extracts against Plasmodium berghei In Vivo.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2018-06-10 eCollection Date: 2018-01-01 DOI:10.1155/2018/2393854

Jackie K Obey, Moses M Ngeiywa, Paul Kiprono, Sabah Omar, Atte von Wright, Jussi Kauhanen, Carina Tikkanen-Kaukanen

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引用次数: 16

Abstract

There is an increasing need for innovative drug and prophylaxis discovery against malaria. The aim of the present study was to test in vivo antiplasmodial activity of Croton macrostachyus H. (Euphorbiaceae) stem bark extracts from Kenyan folkloric medicine. Inbred Balb/c mice were inoculated with erythrocytes parasitized with Plasmodium berghei (ANKA). Different doses (500, 250, and 100 mg/kg) of C. macrostachyus ethyl acetate, methanol, aqueous, and isobutanol extracts were administrated either after inoculation (Peters' 4-day suppressive test) or before inoculation (chemoprotective test) of the parasitized erythrocytes. All the extracts showed significant suppression of parasitemia compared to control (p < 0.001): for the ethyl acetate extract in the range of 58-82%, for the methanol extract in the range of 27-68%, for the aqueous extract in the range of 24-72%, and for the isobutanol extract in the range of 61-80%. Chemoprotective effect was significant (p < 0.001) and the suppression caused by the ethyl acetate extract was between 74 and 100%, by the methanol extract between 57 and 83%, and by the isobutanol extract between 86-92%. The study showed that it is possible to inhibit the growth of the parasites by various stem bark extracts of C. macrostachyus in Balb/c mice supporting the folkloric use of the plant against malaria.

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巴豆茎皮提取物体内抗伯格氏疟原虫活性研究

对疟疾的创新药物和预防发现的需求日益增加。本研究的目的是测试肯尼亚民间医药大戟科(Euphorbiaceae)大戟科(Croton macrostachyus H.)茎皮提取物的体内抗疟原虫活性。用寄生有伯氏疟原虫(Plasmodium berghei, ANKA)的红细胞接种近交系Balb/c小鼠。接种后(彼得斯4天抑制试验)和接种前(化学保护试验)分别给予不同剂量(500、250和100 mg/kg)的巨竹乙酸乙酯提取物、甲醇提取物、水提取物和异丁醇提取物。与对照相比，所有提取物对寄生虫病的抑制作用均显著(p < 0.001):乙酸乙酯提取物的抑制作用范围为58 ~ 82%，甲醇提取物的抑制作用范围为27 ~ 68%，水提物的抑制作用范围为24 ~ 72%，异丁醇提取物的抑制作用范围为61 ~ 80%。化学保护作用显著(p < 0.001)，乙酸乙酯提取物的抑制率为74 ~ 100%，甲醇提取物的抑制率为57 ~ 83%，异丁醇提取物的抑制率为86 ~ 92%。本研究表明，不同种类的大竹叶茎皮提取物均有可能抑制疟原虫在Balb/c小鼠体内的生长，这支持了民间使用大竹叶防治疟疾的说法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.