Hossein Kazemian, Hamid Heidari, Jalil Kardan Yamchi, Hengameh Zandi, Asieh Taji, Fateme Yazdani, Gholamreza Hamzehloo, Roya Ghanavati, Hossein A Rahdar, Mohammad M Feizabadi
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引用次数: 8
Abstract
Background: Mycobacterium tuberculosis as an intracellular pathogen causes Tuberculosis (TB). Due to the long time required for treatment, hepatotoxicity of drugs and also emergence of Multidrug-Resistant (MDR) and Extremely Drug Resistant (XDR) strains, TB is currently a major public health problem. Some medicinal plants possess remarkable activity against Mycobacterium. Among them, Lamiaceae family are of pharmaceutical interest because of their potential antimicrobial properties. The aim of the study was to evaluate the in vitro activities of Satureja rechingeri, Satureja khuzestanica and Zataria multiflora against MDR M. tuberculosis and two Non-Tuberculous Mycobacteria (NTM).
Methods: The essential oils were prepared by the standard method. The confirmed strains were obtained from the microbial collection of Tehran University of Medical Sciences. Minimum Inhibitory Concentrations (MICs) of essential oils of plants against mycobacterial strains were determined using standard broth microdilution method.
Results: MDR M. tuberculosis was completely inhibited by Z. multiflora at 78µg/ml concentration. S. rechingeri and S. khuzestanica also showed same anti-mycobacterial activity against MDR M. tuberculosis with MICs of 156 µg/ml. The MICs of the essential oils against M. tuberculosis H37Rv, M. kansasii and M. fortuitum were in the range from 39 to 156 µg/ml.
Conclusion: The studied medicinal plants showed notable effects against mycobacterial strains. Our results indicated that utilization of Lamiaceae family can be helpful for treatment of mycobacterial infections.
背景:结核分枝杆菌是引起结核病的细胞内病原体。由于治疗时间长，药物具有肝毒性，以及耐多药(MDR)和极耐药(XDR)菌株的出现，结核病目前是一个重大的公共卫生问题。一些药用植物具有显著的抗分枝杆菌活性。其中，Lamiaceae家族因其潜在的抗菌特性而具有药用价值。本研究旨在评价红柳、胡扎柳和扎连对耐多药结核分枝杆菌和两种非结核分枝杆菌(NTM)的体外抑菌活性。方法:采用标准方法制备精油。确认菌株来自德黑兰医科大学的微生物标本。采用标准肉汤微量稀释法测定了植物精油对分枝杆菌菌株的最低抑菌浓度。结果:78µg/ml的多毛菌对MDR M. tuberculosis有完全抑制作用。红曲霉和胡zestanica对耐多药结核分枝杆菌的抑菌活性相同，mic均为156µg/ml。精油对结核分枝杆菌H37Rv、堪萨斯分枝杆菌和福氏分枝杆菌的mic值在39 ~ 156µg/ml之间。结论:所研究的药用植物具有明显的抗分枝杆菌作用。结果表明，利用兰科植物可有效治疗分枝杆菌感染。
期刊介绍：
Recent Patents on Anti-Infective Drug Discovery publishes review articles on recent patents in the field of anti-infective drug discovery e.g. novel bioactive compounds, analogs & targets. A selection of important and recent patents on anti-infective drug discovery is also included in the journal. The journal is essential reading for all researchers involved in anti-infective drug design and discovery.
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