Long-term Culture of Human iPS Cell-derived Telencephalic Neuron Aggregates on Collagen Gel.

IF 2 4区 生物学 Q4 CELL BIOLOGY
Hiroshi Oyama, Koji Takahashi, Yoshikazu Tanaka, Hiroshi Takemoto, Hisashi Haga
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引用次数: 7

Abstract

It takes several months to form the 3-dimensional morphology of the human embryonic brain. Therefore, establishing a long-term culture method for neuronal tissues derived from human induced pluripotent stem (iPS) cells is very important for studying human brain development. However, it is difficult to keep primary neurons alive for more than 3 weeks in culture. Moreover, long-term adherent culture to maintain the morphology of telencephalic neuron aggregates induced from human iPS cells is also difficult. Although collagen gel has been widely used to support long-term culture of cells, it is not clear whether human iPS cell-derived neuron aggregates can be cultured for long periods on this substrate. In the present study, we differentiated human iPS cells to telencephalic neuron aggregates and examined long-term culture of these aggregates on collagen gel. The results indicated that these aggregates could be cultured for over 3 months by adhering tightly onto collagen gel. Furthermore, telencephalic neuronal precursors within these aggregates matured over time and formed layered structures. Thus, long-term culture of telencephalic neuron aggregates derived from human iPS cells on collagen gel would be useful for studying human cerebral cortex development.Key words: Induced pluripotent stem cell, forebrain neuron, collagen gel, long-term culture.

胶原凝胶长期培养人iPS细胞衍生的端脑神经元聚集体。
形成人类胚胎大脑的三维形态需要几个月的时间。因此,建立人类诱导多能干细胞(iPS)神经组织的长期培养方法对研究人类大脑发育具有重要意义。然而,在培养中,原代神经元的存活时间很难超过3周。此外,长期贴壁培养以维持人类iPS细胞诱导的端脑神经元聚集体的形态也很困难。尽管胶原凝胶已被广泛用于支持细胞的长期培养,但目前尚不清楚人类iPS细胞衍生的神经元聚集体能否在这种基质上长期培养。在本研究中,我们将人类iPS细胞分化为端脑神经元聚集体,并检测了这些聚集体在胶原凝胶上的长期培养。结果表明,这些聚集体可以在胶原凝胶上紧密粘附,培养时间超过3个月。此外,这些聚集体中的端脑神经元前体随着时间的推移成熟并形成层状结构。因此,在胶原凝胶上长期培养来自人类iPS细胞的远端脑神经元聚集体将有助于研究人类大脑皮层的发育。关键词:诱导多能干细胞,前脑神经元,胶原凝胶,长期培养
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来源期刊
Cell structure and function
Cell structure and function 生物-细胞生物学
CiteScore
2.50
自引率
0.00%
发文量
6
审稿时长
>12 weeks
期刊介绍: Cell Structure and Function is a fully peer-reviewed, fully Open Access journal. As the official English-language journal of the Japan Society for Cell Biology, it is published continuously online and biannually in print. Cell Structure and Function publishes important, original contributions in all areas of molecular and cell biology. The journal welcomes the submission of manuscripts on research areas such as the cell nucleus, chromosomes, and gene expression; the cytoskeleton and cell motility; cell adhesion and the extracellular matrix; cell growth, differentiation and death; signal transduction; the protein life cycle; membrane traffic; and organelles.
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