Multi-Drug Resistant Clinical Pseudomonas aeruginosas Inhibited by Ferula gummosa Boiss.

Q3 Medicine
Fereshteh Satarian, Hamideh Mahmoodzadeh Hosseini, Abdolamir Ghadaksaz, Mohsen Amin, Abbas Ali Imani Fooladi
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引用次数: 5

Abstract

Background: Multi-drug resistance among Pseudomonas aeruginosa (P. aeruginosa) clinical isolates is increasing and becoming a serious problem for public health authorities worldwide.

Objective: The aim of the current study is to introduce a potent antibacterial compound against the resistant P. aeruginosa.

Methods: In this study, we evaluated the antibacterial effects of extracts and essential oils of Ferula gummosa Boiss (F. gummosa) on 33 P. aeruginosa clinical isolates by microdilution method and assessed the association of antimicrobial activity with the extended spectrum β-lactamase (ESBL) producing, biofilm forming and aliginate production of the strains. In addition, the presence of some genes involved in these properties, including blaGES- 1, blaRER-1, blaCTX-M, blaVEB-1, blaOXA-1, blaOXA-4, blaOXA-10, ppyR, pslA, pelA, algU, algL, algD, fliC and oxaA was determined using PCR.

Results: We revealed that all of our extracts and essential oils had significant antibacterial effects (p<0.001), but the aqueous extracts showed a relatively lower antibacterial activity compared with the methanolic ones. Furthermore, the minimum inhibitory concentration required for the ESBL producing strains was significantly higher than the non-ESBL producing ones (p<0.001). Loss of some genes such as blaPER-1, blaGES-1, blaOXA-1 and blaOXA-4 caused sensitivity to F. gummosa derivatives (p<0.05).

Conclusion: The findings of this study indicate that the antibacterial effects of the extract and essential oils of F. gummosa may be a potential novel treatment against drug-resistant P. aeruginosa clinical isolates.

牙龈阿魏抑制多重耐药临床铜绿假单胞菌。
背景:铜绿假单胞菌(P. aeruginosa)临床分离株的多重耐药性正在增加,并成为全世界公共卫生当局面临的一个严重问题。目的:介绍一种抗耐药铜绿假单胞菌的有效抗菌化合物。方法:本研究采用微稀释法对33株铜绿假单胞菌临床分离株进行了提取物和精油的抑菌效果评价,并评价了抑菌活性与菌株的广谱β-内酰胺酶(ESBL)生成、生物膜形成和海藻酸盐生成的关系。此外,利用PCR方法检测了blaGES- 1、blaRER-1、blaCTX-M、blaVEB-1、blaOXA-1、blaOXA-4、blaOXA-10、ppyR、pslA、pelA、algU、algL、algD、fliC和oxaA等相关基因的存在。结果:所有提取物和挥发油均具有显著的抗菌作用(p)结论:本研究结果表明,甘露假单胞菌提取物和挥发油的抗菌作用可能是治疗耐药铜绿假单胞菌临床分离株的一种潜在的新方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Recent patents on anti-infective drug discovery
Recent patents on anti-infective drug discovery Medicine-Pharmacology (medical)
CiteScore
2.40
自引率
0.00%
发文量
1
期刊介绍: Recent Patents on Anti-Infective Drug Discovery publishes review articles on recent patents in the field of anti-infective drug discovery e.g. novel bioactive compounds, analogs & targets. A selection of important and recent patents on anti-infective drug discovery is also included in the journal. The journal is essential reading for all researchers involved in anti-infective drug design and discovery.
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