[The role of the basophil activation test (BAT) in qualification for specific immunotherapy with inhalant allergens].

Przeglad lekarski Pub Date : 2016-01-01
Małgorzata Bulanda, Wojciech Dyga, Barbara Rusinek, Ewa Czarnobilska
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Abstract

Qualification for specific immunotherapy (SIT) according to the guidelines of the European Academy of Allergy and Clinical Immunology (EAACI) includes medical history, skin prik tests (SPT) and/or measuring the concentration of sIgE. It is necessary to perform additional diagnostic tests in case of discrepancies between the history and the results of SPT/sIgE or differences between SPT and sIgE. Basophil activation test (BAT) assesses the expression of activation markers of these cells, eg. CD63 and CD203c after stimulation. The aim of our study was to evaluate the usefulness of BAT in the qualification for the SIT in comparison to the SPT and sIgE and in case of discrepancies between the results of SPT and sIgE. The study included 30 patients with allergic rhinitis (AR) caused by allergy to house dust mite (Dermatophagoides pteronyssinus, Dp) or birch pollen qualified for SIT. All patients had SPT, sIgE and BAT determination. The group of patients with allergy to birch was a control group for Dp allergic and vice versa. BAT with CD63 antigen expression was performed using a Flow2CAST test. Basophils were stimulated with allergen preparation (50, 500, and 5000 SBU/ml concentrations). BAT results were expressed as a stimulation index (SI). For optimal concentrations of 50 and 500 SBU/ml parameters comparing BAT to SPT and sIgE as the gold standards were consecutively: sensitivity 82-100% and 93-100%, specificity 50-94% and 47-89%, positive predictive value 65- 94% and 61-87%, negative predictive value 86-100% and 93-100%. Correlation BAT - SPT and BAT - sIgE ranged within 0.59 to 0.84 and 0.51 to 0.72. BAT was helpful in 2 of 30 patients with incompatible results of SPT and sIgE. Optimal concentrations for basophil stimulation are 50 and 500 SBU/ ml. BAT may be useful diagnostic tool in the qualification for the SIT in case of discrepancies between the results of SPT and sIgE.

[嗜碱性粒细胞激活试验(BAT)在吸入性过敏原特异性免疫治疗鉴定中的作用]。
根据欧洲过敏和临床免疫学学会(EAACI)的指导方针,特异性免疫治疗(SIT)的资格包括病史、皮肤刺痛试验(SPT)和/或sIgE浓度的测量。如果SPT/sIgE的病史和结果不一致,或SPT和sIgE之间存在差异,则有必要进行额外的诊断试验。嗜碱性活化试验(BAT)评估这些细胞的活化标记物的表达。刺激后CD63和CD203c。我们研究的目的是评估bat与SPT和sIgE的比较,以及在SPT和sIgE结果之间存在差异的情况下,对SIT资格的有用性。该研究包括30例过敏性鼻炎(AR)患者,这些患者是由对屋尘螨(Dermatophagoidespteronyssinus, Dp)或符合SIT条件的桦树花粉过敏引起的。所有患者均行SPT、sIgE和BAT测定。以桦树过敏组为Dp过敏对照组,以桦树过敏组为Dp过敏对照组,采用flow2cast法检测CD63抗原表达的bat。用过敏原制剂(50,500和5000 SBU/ml浓度)刺激嗜碱性细胞。bat结果以刺激指数(SI)表示。对于50和500 SBU/ml的最佳浓度,将BAT与spt和sIgE作为金标准的参数依次为:灵敏度82-100%和93-100%,特异性50-94%和47-89%,阳性预测值65-94%和61-87%,阴性预测值86-100%和93-100%。BAT - SPT和BAT - sIgE的相关性在0.59 ~ 0.84和0.51 ~ 0.72之间,在30例SPT和sIgE结果不一致的患者中,BAT对2例有帮助。嗜碱性刺激的最佳浓度为50和500 SBU/ml。在SPT和sIgE结果不一致的情况下,BAT可能是SIT资格的有用诊断工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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