Secretory immunoglobulin A concentration in non-stimulated and stimulated saliva in relation to the status of smoking.

Przeglad lekarski Pub Date : 2016-01-01
Marta Nakonieczna-Rudnicka, Teresa Bachanek, Wanda Piekarczyk, Elżbieta Kobyłecka
{"title":"Secretory immunoglobulin A concentration in non-stimulated and stimulated saliva in relation to the status of smoking.","authors":"Marta Nakonieczna-Rudnicka,&nbsp;Teresa Bachanek,&nbsp;Wanda Piekarczyk,&nbsp;Elżbieta Kobyłecka","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Secretory immunoglobulin\nA (sIgA) is a dominant immunoglobulin\nin the saliva. It is the first\nline of defense against microorganisms.</p><p><strong>Aim of the study: </strong>Analysis of secretory\nimmunoglobulin A concentration\nin non-stimulated and stimulated\nsaliva. Assessment of sIgA concentration\nin relation to the status of cigarette\nsmoking of the investigated.</p><p><strong>Material and methods: </strong>Survey and biochemical studies of saliva were\nconducted in the group of 109 people\n(smokers and non-smokers) aged\n20-54. The smokers smoked from\n5 to more than 20 cigarettes daily.\nThe investigation material was nonstimulated\nand stimulated saliva collected\nfrom patients on the same day\nbetween 9.30 and 11.30 a.m, 1.5-2h\nafter meal. Directly after non-stimulated\nand stimulated saliva collection\nSalivette tubes were placed in the ice\ncontainer with the temperature of 4oC,\nthen centrifuged at the temperature\nof 4oC for 12 minutes at 3000 r/min.\nThe obtained supernatant was stored\nat the temperature of -75ºC until the\nassays were performed. sIgA concentration\nwas determined using sIgA\nELISA Kit (Immunodiagnostik AG,\nGermany). Statistical analysis was\nconducted with the use of Mann –\nWhitney test. While investigating the\ninfluence of age on the studied parameters\nSpearman correlation coefficient\nand its significance were used.\nStatistically significant test values\nwere those of p<0.05.</p><p><strong>Results: </strong>In non-stimulated saliva\nsIgA concentration was significantly\nhigher compared to stimulated saliva\n(Z = 4.00, p<0.001). No significant differences\nwere stated in sIgA concentration\nin non-stimulated saliva between\nsmokers and non-smokers (Z =\n0.26, p>0.05). No essential differences\nwere stated in sIgA concentration in\nstimulated saliva between non-smokers\nand smokers (Z = 0.23, p>0.05).\nEssential differences were stated between\nthe groups. In men sIgA concentration in stimulated\nsaliva was significantly higher compared to women (Z =\n2.25, p<0.05).</p><p><strong>Conclusions: </strong>sIgA concentration in non-stimulated\nsaliva is significantly higher in comparison to stimulated\nsaliva. The status of cigarette smoking does not influence\nthe essential differences in sIgA concentration in nonstimulated\nand stimulated saliva. In men sIgA concentration\nin stimulated saliva is significantly higher in comparison\nto women.</p>","PeriodicalId":21148,"journal":{"name":"Przeglad lekarski","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Przeglad lekarski","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Introduction: Secretory immunoglobulin A (sIgA) is a dominant immunoglobulin in the saliva. It is the first line of defense against microorganisms.

Aim of the study: Analysis of secretory immunoglobulin A concentration in non-stimulated and stimulated saliva. Assessment of sIgA concentration in relation to the status of cigarette smoking of the investigated.

Material and methods: Survey and biochemical studies of saliva were conducted in the group of 109 people (smokers and non-smokers) aged 20-54. The smokers smoked from 5 to more than 20 cigarettes daily. The investigation material was nonstimulated and stimulated saliva collected from patients on the same day between 9.30 and 11.30 a.m, 1.5-2h after meal. Directly after non-stimulated and stimulated saliva collection Salivette tubes were placed in the ice container with the temperature of 4oC, then centrifuged at the temperature of 4oC for 12 minutes at 3000 r/min. The obtained supernatant was stored at the temperature of -75ºC until the assays were performed. sIgA concentration was determined using sIgA ELISA Kit (Immunodiagnostik AG, Germany). Statistical analysis was conducted with the use of Mann – Whitney test. While investigating the influence of age on the studied parameters Spearman correlation coefficient and its significance were used. Statistically significant test values were those of p<0.05.

Results: In non-stimulated saliva sIgA concentration was significantly higher compared to stimulated saliva (Z = 4.00, p<0.001). No significant differences were stated in sIgA concentration in non-stimulated saliva between smokers and non-smokers (Z = 0.26, p>0.05). No essential differences were stated in sIgA concentration in stimulated saliva between non-smokers and smokers (Z = 0.23, p>0.05). Essential differences were stated between the groups. In men sIgA concentration in stimulated saliva was significantly higher compared to women (Z = 2.25, p<0.05).

Conclusions: sIgA concentration in non-stimulated saliva is significantly higher in comparison to stimulated saliva. The status of cigarette smoking does not influence the essential differences in sIgA concentration in nonstimulated and stimulated saliva. In men sIgA concentration in stimulated saliva is significantly higher in comparison to women.

分泌性免疫球蛋白A在非刺激和刺激唾液中的浓度与吸烟状态的关系。
分泌性免疫球蛋白a (sIgA)是唾液中的显性免疫球蛋白。它是抵御微生物的第一道防线。研究目的:分析非刺激和刺激唾液中分泌免疫球蛋白A的浓度。评估sIgA浓度与被调查者吸烟状况的关系。材料与方法:对年龄在20-54岁的109人(吸烟者和非吸烟者)进行唾液调查和生化研究。吸烟者每天抽5到20多支烟。调查材料是在当天上午9:30 - 11:30,饭后1.5-2点之间从患者身上采集的非刺激和刺激唾液。非刺激和刺激唾液采集后,直接将唾液管置于温度为4oC的冰容器中,然后在温度为4oC的条件下,以3000 r/min离心12分钟。将获得的上清液保存在-75℃的温度下,直到进行检测。sIgA浓度采用sIgAELISA Kit (immunodiagnostic AG,Germany)测定。采用Mann -Whitney检验进行统计分析。在研究年龄对研究参数的影响时,采用spearman相关系数及其显著性。结果:未刺激唾液中iga浓度显著高于刺激唾液(Z = 4.00, p0.05)。非吸烟者和吸烟者唾液中sIgA浓度无本质差异(Z = 0.23, p>0.05)。指出了两组之间的本质差异。男性受刺激唾液中的sIgA浓度明显高于女性(Z =2.25)。结论:未受刺激唾液中的sIgA浓度明显高于受刺激唾液。吸烟状况不影响未受刺激和受刺激唾液中sIgA浓度的本质差异。男性受刺激唾液中的sIgA浓度明显高于女性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信