[Microbiological diagnostics of infective endocarditis in the light of the new guidelines of the European Society of Cardiology with particular focus on the molecular methods].
Malgorzata Małek-Elikowska, Waldemar Elikowski, Monika Lisiecka, Andrzej Szyszka
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引用次数: 0
Abstract
The authors describe the presentday
possibilities of routine and molecular
microbiologic diagnostics of
infective endocarditis (IE). Routine
diagnostics employs automated microbial
growth and biochemical detection
systems. Molecular methods are based
on polymerase chain reaction (PCR)
and matrix assisted laser desorption/
ionization time of flight mass spectrometry
(MALDI-TOF MS). MALDI-TOF
MS first appeared in the new guidelines
for the management of IE of the European
Society of Cardiology published
in 2015. The greatest benefit of MALDITOF
MS is the short time of pathogen
identification. The main disadvantages
are the necessity of routine agar cultures
and lack of antimicrobial susceptibility
estimation. So far, there
has been no Polish literature report
on MALDI-TOF MS used for pathogen
identification in suspected IE. This may
well result from scarce accessibility of
the method in Poland. The paper aims
to stress the importance of an up-todate
technology in the diagnostics of
difficult, blood culture negative cases
of IE. It also takes into consideration
the limitations of mass spectrometry
in the ESC diagnostic scheme of IE.