[Microbiological diagnostics of infective endocarditis in the light of the new guidelines of the European Society of Cardiology with particular focus on the molecular methods].

Przeglad lekarski Pub Date : 2016-01-01
Malgorzata Małek-Elikowska, Waldemar Elikowski, Monika Lisiecka, Andrzej Szyszka
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Abstract

The authors describe the presentday possibilities of routine and molecular microbiologic diagnostics of infective endocarditis (IE). Routine diagnostics employs automated microbial growth and biochemical detection systems. Molecular methods are based on polymerase chain reaction (PCR) and matrix assisted laser desorption/ ionization time of flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS first appeared in the new guidelines for the management of IE of the European Society of Cardiology published in 2015. The greatest benefit of MALDITOF MS is the short time of pathogen identification. The main disadvantages are the necessity of routine agar cultures and lack of antimicrobial susceptibility estimation. So far, there has been no Polish literature report on MALDI-TOF MS used for pathogen identification in suspected IE. This may well result from scarce accessibility of the method in Poland. The paper aims to stress the importance of an up-todate technology in the diagnostics of difficult, blood culture negative cases of IE. It also takes into consideration the limitations of mass spectrometry in the ESC diagnostic scheme of IE.

[根据欧洲心脏病学会新指南,感染性心内膜炎的微生物诊断,特别关注分子方法]。
作者描述了目前常规和分子微生物诊断有效心内膜炎(IE)的可能性。常规诊断采用自动化微生物生长和生化检测系统。分子方法是基于聚合酶链反应(PCR)和基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)。MALDI-TOFMS首次出现在2015年发布的欧洲心脏病学会IE管理新指南中。MALDITOFMS最大的优点是病原鉴定时间短。其主要缺点是需要常规琼脂培养和缺乏抗菌药物敏感性评估。到目前为止,波兰尚未见MALDI-TOF MS用于疑似IE病原菌鉴定的文献报道。这很可能是由于波兰缺乏这种方法的可及性。本文旨在强调一项最新技术在诊断困难的、血培养阴性的IE病例中的重要性。同时也考虑到了质谱法在ESC诊断方案中的局限性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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