Infrared Laser Improves Collagen Organization in Muscle and Tendon Tissue During the Process of Compensatory Overload.

Q2 Medicine

Photomedicine and laser surgery Pub Date : 2018-03-01 Epub Date: 2018-02-21 DOI:10.1089/pho.2017.4302

Stella Maris Lins Terena, Kristianne Porta Santos Fernandes, Sandra Kalil Bussadori, Aldo Brugnera Junior, Daniela de Fátima Teixeira da Silva, Eric Moreno Ramos Magalhães, Raquel Agnelli Mesquita Ferrari

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引用次数: 7

Abstract

Background: The photobiomodulation using the low-level laser therapy (LLLT) exerts a positive modulating effect on the synthesis of collagen in skeletal muscles and tendons. However, few studies have addressed this effect during the compensatory overload.

Objective: Evaluate the effect of infrared laser on the deposition and organization of collagen fibers in muscle and tendon tissue during compensatory overload of the plantar muscle in rats.

Materials and methods: Wistar rats were submitted to bilateral ablation of the synergist muscles of the hind paws and divided in groups: Control, Hypertrophy, and Hypertrophy (H)+LLLT (780 nm, 40 mW, 9.6 J/cm² and 10 s/point, 8 points, total energy 3.2 J, daily), evaluated at 7 and 14 days. Muscle cuts were stained with Picrosirius-Red and hematoxylin-eosin and tendon cuts were submitted to birefringence for determination of collagen distribution and organization.

Results: After 7 days an increase was observed in the area between beam muscles in H+LLLT (25.45% ± 2.56) in comparison to H (20.3% ± 3.31), in mature fibers and fibrilis in H+LLLT (29346.88 μm² ± 2182.56; 47602.8 μm² ± 2201.86 respectively) in comparison to H (26656.5 μm² ± 1880.46; 45630.34 μm² ± 2805.82 respectively) and in the collagen area in H+LLLT (2.25% ± 0.19) in comparison to H (2.0% ± 0.15). However, after 14 days a reduction was observed in the area between beam muscles in H+LLLT (13.88% ± 2.54) in comparison to H (19.1% ± 2.61), in fibrils and mature fibers in H+LLLT (17174.1 μm² ± 2563.82; 32634.04 μm² ± 1689.38 respectively) in comparison to H (55249.86 μm² ± 1992.65; 44318.36 μm² ± 1759.57) and in the collagen area in H+LLLT (1.76% ± 0.16) in comparison to H (2.09 ± 0.27). A greater organization of collagen fibers in the tendon was observed after 7 and 14 days in H+LLLT groups.

Conclusions: Infrared laser irradiation induces an improvement in collagen organization in tendons and a reduction in the total area of collagen in muscles during compensatory atrophy following the ablation of synergist muscles.

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红外激光改善代偿超负荷过程中肌肉和肌腱组织中的胶原蛋白组织。

背景:低强度激光治疗(LLLT)对骨骼肌和肌腱中胶原蛋白的合成具有正向调节作用。然而，很少有研究涉及代偿性过载期间的这种影响。目的:探讨红外激光对大鼠足底肌代偿性过载时肌肉和肌腱组织中胶原纤维沉积和组织的影响。材料与方法:Wistar大鼠双侧后爪增效肌消融，分为对照组、肥厚组和肥厚(H)+LLLT组(780 nm, 40 mW, 9.6 J/cm2, 10 s/点，8点，总能量3.2 J，每日)，于7天和14天进行评估。肌肉切口用Picrosirius-Red和苏木精-伊红染色，肌腱切口用双折射法测定胶原的分布和组织。结果:7 d后，H+LLLT的梁肌间面积(25.45%±2.56)比H(20.3%±3.31)增加，成熟纤维和原纤维(29346.88 μm2±2182.56);分别为47602.8 μm2±2201.86)，而H (26656.5 μm2±1880.46;(45630.34 μm2±2805.82)，H+LLLT胶原区(2.25%±0.19)比H(2.0%±0.15)。然而，14天后，与H(19.1%±2.61)相比，H+LLLT的梁肌间面积减少了13.88%±2.54，原纤维和成熟纤维减少了17174.1 μm2±2563.82;32634.04 μm2±1689.38)，而H (55249.86 μm2±1992.65;(44318.36 μm2±1759.57)，H+LLLT胶原区(1.76%±0.16)比H(2.09±0.27)。第7天和第14天，H+LLLT组肌腱中胶原纤维的组织更大。结论:在增效肌消融后的代偿性萎缩过程中，红外激光照射诱导肌腱胶原组织的改善和肌肉胶原总面积的减少。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Photomedicine and laser surgery 医学-外科

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Photobiomodulation, Photomedicine, and Laser Surgery (formerly Photomedicine and Laser Surgery) is the essential journal for cutting-edge advances and research in phototherapy, low-level laser therapy (LLLT), and laser medicine and surgery. The Journal delivers basic and clinical findings and procedures to improve the knowledge and application of these techniques in medicine.