Amin Allah Dashtiyan , Masood Sepehrimanesh , Nader Tanideh , Mohammad Esmaeil Afzalpour
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引用次数: 6
Abstract
The aim of this study was to investigate the effect of endurance training with and without vitamin E on the expression of p53 and Phosphatase and tension homolog (PTEN) tumor suppressor genes of prostate glands in male rats. For this purpose, 50 Sprague–Dawley male rats were randomly assigned into 5 groups: (1) control group (CON, n = 10), (2) sham (S, n = 10), (3) endurance training (ET, n = 10), (4) endurance training + vitamin E (ET + VE, n = 10), (5) vitamin E (VE, n = 10). Endurance training protocol was implemented for 6 weeks, 6 days per week, in accordance with the overload principle. To measure expression changes of p53 and PTEN genes in rats' prostate, real-time PCR method was used and HPLC method was used to measure vitamin E in this tissue. After 6 weeks of taking vitamin E, its level in all groups, except for group VE (p < 0.000) did not significantly increase. After implementing training protocol, p53 expression reduced significantly in ET group (p < 0.026). Vitamin E supplementation along with endurance training did not cause any significant change either p53 or PTEN (respectively; p < 0.2, p < 0.11). Instead, vitamin E supplementation without endurance training caused significant increase in PTEN, but did not cause any significant changes in p53 (respectively; p < 0.016, p < 0.15). These results indicate that endurance training reduces p53 and PTEN tumor suppressing genes expression, and taking vitamin E supplement could increase expression of these genes in some extent.
本研究旨在探讨耐力训练加维生素E和不加维生素E对雄性大鼠前列腺p53、磷酸酶和张力同源(PTEN)肿瘤抑制基因表达的影响。为此,将50只雄性Sprague-Dawley大鼠随机分为5组:(1)对照组(CON, n = 10),(2)假手术组(S, n = 10),(3)耐力训练组(ET, n = 10),(4)耐力训练+维生素E (ET + VE, n = 10),(5)维生素E (VE, n = 10)。耐力训练方案实施6周,每周6天,按照过载原则。采用实时荧光定量PCR法测定大鼠前列腺组织中p53和PTEN基因的表达变化,HPLC法测定大鼠前列腺组织中维生素E的含量。服用维生素E 6周后,除VE组外,其余各组维生素E水平均显著高于对照组(p