Prevalence of Gram-Negative Bacilli Isolated from the Equipment and Surfaces in Hospital Wards of Golestan Province, North of Iran.

European Journal of Microbiology & Immunology Pub Date : 2017-12-04 eCollection Date: 2017-12-18 DOI:10.1556/1886.2017.00015
Ali Asghar Ayatollahi, Abolfazl Amini, Somayeh Rahimi, Saeid Rahbar Takrami, Reza Kazemi Darsanaki, Muhammad Sadeqi Nezhad
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引用次数: 10

Abstract

Nosocomial infections are one of the most common causes of death in hospitals. This study aimed to determine the prevalence of gram-negative bacilli isolated from the equipment in hospital wards of the Golestan province, in the year 2015. In this cross-sectional study in 2015, 1980 samples from medical and nonmedical equipment and surfaces were collected from the wards of 13 teaching hospitals, in the Golestan province. Samples were inoculated into eosin methylene blue agar and blood agar culture media and isolated colonies were identified by standard biochemical tests. The obtained results were then analyzed using SPSS 22 software and χ2 test. Among 1980 isolated samples, 601 samples (30.35%) were infected with gram-negative bacilli while Enterobacter aerogenes (37.27%) was responsible for most of the contaminations. The highest rate of infection was observed in the intensive care unit (33.1%), and the highest level of contamination in the medical equipment was associated with laryngoscope and its blade (10.48%), as well as ECG sensor and its monitoring connector (6.65%). Meanwhile, phone (6.32%) and patients' beds and linen (5.15%) had the highest level of contamination in the nonmedical equipment. Considering the high rates of gram-negative bacilli contamination in the hospital wards of the Golestan province, thorough hand washing as the main action for disinfection and sterilizing the equipment, as well as performing periodic cultivation alongside the use of standard guidelines for prevention and control of nosocomial infections, are recommended to reduce the level of contamination.

伊朗北部戈列斯坦省医院病房设备和表面分离的革兰氏阴性杆菌流行率
院内感染是院内最常见的死亡原因之一。本研究旨在确定2015年戈列斯坦省医院病房设备中分离的革兰氏阴性杆菌的流行情况。在2015年的这项横断面研究中,从Golestan省13家教学医院的病房收集了1980个医疗和非医疗设备和表面样本。将样品接种于伊红亚甲基蓝琼脂和血琼脂培养基中,通过标准生化试验鉴定分离菌落。采用SPSS 22软件和χ2检验对所得结果进行分析。1980份分离标本中,革兰氏阴性杆菌感染601份(30.35%),其中产气肠杆菌感染最多(37.27%)。重症监护病房感染率最高(33.1%),医疗设备污染水平最高的是喉镜及其刀片(10.48%)、心电传感器及其监测连接器(6.65%)。非医疗设备中,手机(6.32%)和病人床和床单(5.15%)污染程度最高。考虑到戈列斯坦省医院病房的革兰氏阴性杆菌污染率很高,建议将彻底洗手作为设备消毒和灭菌的主要行动,并在使用预防和控制医院感染的标准准则的同时进行定期培养,以降低污染水平。
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