Bioanalytical applications of surface-enhanced Raman spectroscopy: de novo molecular identification.

IF 3.6 3区 化学 Q2 CHEMISTRY, ANALYTICAL
Reviews in Analytical Chemistry Pub Date : 2017-12-01 Epub Date: 2017-07-05 DOI:10.1515/revac-2016-0037
Anh H Nguyen, Emily A Peters, Zachary D Schultz
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引用次数: 26

Abstract

Surface enhanced Raman scattering (SERS) has become a powerful technique for trace analysis of biomolecules. The use of SERS-tags has evolved into clinical diagnostics, the enhancement of the intrinsic signal of biomolecules on SERS active materials shows tremendous promise for the analysis of biomolecules and potential biomedical assays. The detection of the de novo signal from a wide range of biomolecules has been reported to date. In this review, we examine different classes of biomolecules for the signals observed and experimental details that enable their detection. In particular, we survey nucleic acids, amino acids, peptides, proteins, metabolites, and pathogens. The signals observed show that the interaction of the biomolecule with the enhancing nanostructure has a significant influence on the observed spectrum. Additional experiments demonstrate that internal standards can correct for intensity fluctuations and provide quantitative analysis. Experimental methods that control the interaction at the surface are providing for reproducible SERS signals. Results suggest that combining advances in methodology with the development of libraries for SERS spectra may enable the characterization of biomolecules complementary to other existing methods.

Abstract Image

Abstract Image

Abstract Image

表面增强拉曼光谱的生物分析应用:从头分子鉴定。
表面增强拉曼散射(SERS)已成为生物分子痕量分析的一种强有力的技术。SERS标签的使用已经发展到临床诊断,SERS活性材料上生物分子固有信号的增强为生物分子分析和潜在的生物医学分析显示了巨大的前景。迄今为止,从广泛的生物分子中检测新生信号已被报道。在这篇综述中,我们检查不同类别的生物分子的信号观察和实验细节,使他们的检测。特别是,我们调查核酸,氨基酸,多肽,蛋白质,代谢物和病原体。观察到的信号表明,生物分子与增强纳米结构的相互作用对观察到的光谱有显著影响。另外的实验表明,内标可以校正强度波动并提供定量分析。控制表面相互作用的实验方法提供了可重复的SERS信号。结果表明,将方法的进步与SERS光谱文库的开发相结合,可以使生物分子的表征与其他现有方法相补充。
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来源期刊
Reviews in Analytical Chemistry
Reviews in Analytical Chemistry 化学-分析化学
CiteScore
7.50
自引率
0.00%
发文量
15
审稿时长
>12 weeks
期刊介绍: Reviews in Analytical Chemistry publishes authoritative reviews by leading experts in the dynamic field of chemical analysis. The subjects can encompass all branches of modern analytical chemistry such as spectroscopy, chromatography, mass spectrometry, electrochemistry and trace analysis and their applications to areas such as environmental control, pharmaceutical industry, automation and other relevant areas. Review articles bring the expert up to date in a concise manner and provide researchers an overview of new techniques and methods.
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