Articular cartilage response to a sliding load using two different-sized spherical indenters1.

IF 1 4区医学 Q4 BIOPHYSICS

Biorheology Pub Date : 2018-01-01 DOI:10.3233/BIR-16110

Oliver R Schätti, Vera Colombo, Peter A Torzilli, Luigi M Gallo

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引用次数: 5

Abstract

Background: Cartilage surface contact geometry influences the deformational behavior and stress distribution throughout the extracellular matrix (ECM) under load.

Objective: To test the correlation between the mechanical and cellular response of articular cartilage when loaded with two different-sized spherical indenters under dynamic reciprocating sliding motion.

Methods: Articular cartilage explants were subjected to a reciprocating sliding load using a 17.6 mm or 30.2 mm spherical ball for 2000 cycles at 10 mm/s and 4 kg axial load. Deformation of the cartilage was recorded and contact parameters were calculated according to Hertzian theory. After mechanical loading cartilage samples were collected and analyzed for ECM collagen damage, gene regulation and proteoglycan (PG) loss.

Results: Significantly higher ECM deformation and strain and lower dynamic effective modulus were found for explants loaded with the smaller diameter indenter whereas contact radius and stress remained unaffected. Also, the 17.6 mm indenter increased PG loss and significantly upregulated genes for ECM proteins and enzymes as compared to the 30.2 mm indenter.

Conclusion: Sliding loads that increase ECM deformation/strain were found to induce enzyme-mediated catabolic processes in articular cartilage explants. These observations provide further understanding of how changes in cartilage contact mechanics under dynamic conditions can affect the cellular response.

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用两个不同大小的球形压头观察关节软骨对滑动载荷的反应。

背景:软骨表面接触几何形状影响细胞外基质(ECM)在载荷作用下的变形行为和应力分布。目的:研究两种不同大小的球形压头在动态往复滑动运动下关节软骨力学反应与细胞反应的相关性。方法:采用17.6 mm或30.2 mm的球形球，以10 mm/s和4 kg的轴向载荷对关节软骨进行往复滑动加载，共2000次。记录软骨的变形，并根据赫兹理论计算接触参数。机械加载后收集软骨样品，分析ECM胶原损伤、基因调控和蛋白聚糖(PG)损失。结果:在接触半径和应力不受影响的情况下，较小直径压头加载的外植体的ECM变形和应变显著增加，动态有效模量显著降低。此外，与30.2 mm的压痕相比，17.6 mm的压痕增加了PG的损失，并显著上调了ECM蛋白和酶的基因。结论:发现滑动载荷增加ECM变形/应变可诱导关节软骨外植体中酶介导的分解代谢过程。这些观察结果为进一步了解动态条件下软骨接触力学的变化如何影响细胞反应提供了依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biorheology 医学-工程：生物医学

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biorheology is an international interdisciplinary journal that publishes research on the deformation and flow properties of biological systems or materials. It is the aim of the editors and publishers of Biorheology to bring together contributions from those working in various fields of biorheological research from all over the world. A diverse editorial board with broad international representation provides guidance and expertise in wide-ranging applications of rheological methods to biological systems and materials. The scope of papers solicited by Biorheology extends to systems at different levels of organization that have never been studied before, or, if studied previously, have either never been analyzed in terms of their rheological properties or have not been studied from the point of view of the rheological matching between their structural and functional properties. This biorheological approach applies in particular to molecular studies where changes of physical properties and conformation are investigated without reference to how the process actually takes place, how the forces generated are matched to the properties of the structures and environment concerned, proper time scales, or what structures or strength of structures are required.