In vitro measurement of platelet adhesion to intact endothelial cells under low shear conditions.

IF 1 4区医学 Q4 BIOPHYSICS

Biorheology Pub Date : 2018-01-01 DOI:10.3233/BIR-17139

Yuki Ogawa, Takuma Nakahara, Yuma Nishida, Chihiro Kobayashi, Takamichi Hirata, Hideaki Nagano, Suguru Shiratori, Kenjiro Shimano

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引用次数: 2

Abstract

Background: Prediction of thrombus formation at intact arterial walls under low shear flow conditions is clinically important particularly for better prognoses of embolisation in cerebral aneurysms. Although a new mathematical model for this purpose is necessary, little quantitative information has been known about platelet adhesion to intact endothelial cells.

Objective: The objective of this study is to measure the number of platelets adhering to intact endothelial cells with a focus upon the influence of the shear rate.

Methods: Endothelial cells disseminated in μ-slides were exposed to swine whole blood at different shear rates. Adenosine diphosphate (ADP) was used as an agonist. Adherent platelets were counted by means of scanning electron microscopy.

Results: At an ADP concentration of 1 µM, 20.8 ± 3.1 platelets per 900 µm2 were observed after 30-minute perfusion at a shear rate of 0.8 s-1 whereas only 3.0 ± 1.4 per 900 µm2 at 16.8 s-1.

Conclusions: The number of adherent platelets is determined by a balance between the shear and the degree of stimulation by the agonist. At an ADP concentration of 1 µM, a limit to the shear rate at which platelets can adhere to intact endothelial cells is considered to be slightly higher than 16.8 s-1.

查看原文本刊更多论文

低剪切条件下血小板粘附完整内皮细胞的体外测定。

背景:低剪切血流条件下完整动脉壁血栓形成的预测在临床上具有重要意义，特别是对脑动脉瘤栓塞的预后更好。尽管为此目的建立一个新的数学模型是必要的，但关于血小板粘附完整内皮细胞的定量信息知之甚少。目的:本研究的目的是测量血小板粘附完整内皮细胞的数量，重点关注剪切速率的影响。方法:以不同的剪切速率将分布于μ玻片上的内皮细胞暴露于猪全血中。二磷酸腺苷(ADP)作为激动剂。用扫描电镜对粘附血小板进行计数。结果:ADP浓度为1µM时，在0.8 s-1剪切速率下，灌注30分钟后，每900µm2可观察到20.8±3.1个血小板，而在16.8 s-1剪切速率下，每900µm2仅观察到3.0±1.4个血小板。结论:粘附血小板的数量取决于受体激动剂的剪切和刺激程度之间的平衡。在ADP浓度为1µM时，血小板粘附完整内皮细胞的剪切速率极限被认为略高于16.8 s-1。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biorheology 医学-工程：生物医学

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biorheology is an international interdisciplinary journal that publishes research on the deformation and flow properties of biological systems or materials. It is the aim of the editors and publishers of Biorheology to bring together contributions from those working in various fields of biorheological research from all over the world. A diverse editorial board with broad international representation provides guidance and expertise in wide-ranging applications of rheological methods to biological systems and materials. The scope of papers solicited by Biorheology extends to systems at different levels of organization that have never been studied before, or, if studied previously, have either never been analyzed in terms of their rheological properties or have not been studied from the point of view of the rheological matching between their structural and functional properties. This biorheological approach applies in particular to molecular studies where changes of physical properties and conformation are investigated without reference to how the process actually takes place, how the forces generated are matched to the properties of the structures and environment concerned, proper time scales, or what structures or strength of structures are required.