Effects of partial dietary supplementation of fish meal with soymeal on the stress and apoptosis response in the digestive system of common dentex (Dentex dentex).

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2017-12-21 eCollection Date: 2017-12-01 DOI:10.1186/s40709-017-0071-1

Efthimia Antonopoulou, Eleni Chouri, Konstantinos Feidantsis, Antigone Lazou, Stavros Chatzifotis

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引用次数: 1

Abstract

Background: Soybean is a common alternative protein source of plant origin in aquafeeds as it has a reasonably balanced amino acid profile and is widely available. This study aimed to investigate the influence of partial substitution of fish meal with soy meal on cytoprotective pathways and apoptosis in the digestive system of common dentex (Dentex dentex), using the activation of Hsp70, p38 MAPK, Bcl-2 and caspase-3. The experimental approach involved feeding of common dentex with three isoprotein and isoenergetic diets that contained fish meal as a protein source (FM), partial replacement of fish meal by soy meal 25% (SM25) and 40% (SM40) for 3 months.

Results: The SM40 diet induced Hsp70 activation only in the middle part of intestine. On the other hand, both SM25 and SM40 diets diminished the phosphorylation of p38 MAPK in the anterior and the middle part of intestine, whereas only SM25 induced p38 MAPK phosphorylation in the stomach. Moreover, a decrease in the levels of caspase-3 activity was observed in the middle and posterior intestine, as well as in the stomach after feeding with SM25 diets. Furthermore, Bcl-2 levels were increased by SM40 in the anterior and by SM25 in the middle part of intestine.

Conclusions: SM25 and SM40 diets elicited a tissue and soy concentration specific cellular and cell protective response in the different parts of the digestive tract in common dentex.

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饲料中添加部分鱼粉和豆粕对普通齿鲆消化系统应激和细胞凋亡反应的影响。

背景:大豆是水产饲料中常见的植物性替代蛋白质来源，因为它具有合理平衡的氨基酸结构，并且广泛使用。本研究旨在通过激活Hsp70、p38 MAPK、Bcl-2和caspase-3，探讨豆粕部分替代鱼粉对普通牙(dentex dentex)消化系统细胞保护通路和细胞凋亡的影响。试验方法是用三种以鱼粉为蛋白质来源(FM)的等蛋白和等能饲料饲喂共同齿，用25% (SM25)和40% (SM40)的豆粕部分替代鱼粉，为期3个月。结果:SM40日粮仅在肠道中部诱导Hsp70活化。另一方面，SM25和SM40饮食都降低了肠道前部和中部p38 MAPK的磷酸化，而只有SM25诱导了胃中p38 MAPK的磷酸化。此外，在喂食SM25日粮后，在中肠和后肠以及胃中观察到caspase-3活性水平下降。Bcl-2水平在肠前部增加SM40，在肠中部增加SM25。结论:SM25和SM40日粮在大鼠共齿消化道不同部位引起了组织和大豆浓度特异性的细胞和细胞保护反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.