Magnesium enhances the beneficial effects of NK1 antagonist administration on blood-brain barrier permeability and motor outcome after traumatic brain injury.

IF 1.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Joshua L Ameliorate, Mounir N Ghabriel, Robert Vink
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引用次数: 13

Abstract

The current study investigated whether adding magnesium to an NK1 tachykinin receptor antagonist after traumatic brain injury would enhance efficacy to further reduce blood-brain barrier permeability and improve functional recovery compared to either treatment alone. Sprague-Dawley rats were injured using the impact acceleration model of diffuse brain injury, and received either no treatment, MgSO4 (30 mg/kg IV), the NK1 antagonist n-acetyl L tryptophan (2.5 mg/kg IP), or both agents combined. Animals were then killed at either 1, 5, or 24 h postinjury for determination of blood-brain barrier permeability using previously administered Evans blue dye or assessed for functional outcome over a 1-week period using the rotarod motor test. As expected, both MgSO4 and n-acetyl L tryptophan significantly reduced blood-brain barrier permeability and improved functional outcome. However, combined n-acetyl L tryptophan and MgSO4 was more effective at reducing blood-brain barrier permeability (P < 0.05) and improving functional outcome (P < 0.001) compared to the individual compounds. Our results demonstrate that combination therapy with magnesium and an NK1 antagonist may be a more effective therapy for TBI than either compound administered alone.

镁增强了NK1拮抗剂对创伤性脑损伤后血脑屏障通透性和运动预后的有益作用。
本研究探讨了创伤性脑损伤后,与单独治疗相比,在NK1速激肽受体拮抗剂中加入镁是否能增强进一步降低血脑屏障通透性和改善功能恢复的疗效。Sprague-Dawley大鼠采用弥漫性脑损伤冲击加速度模型,分别给予不给药、MgSO4 (30 mg/kg IV)、NK1拮抗剂n-乙酰L色氨酸(2.5 mg/kg IP)或两种药物联合治疗。然后在伤后1、5或24小时处死动物,用先前给药的埃文斯蓝染料测定血脑屏障通透性,或用旋转杆运动试验评估1周内的功能结果。正如预期的那样,MgSO4和n-乙酰L色氨酸都显著降低了血脑屏障的通透性,改善了功能结果。然而,n-乙酰L色氨酸和MgSO4联合使用更有效地降低血脑屏障的通透性(P
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Magnesium research
Magnesium research 医学-内分泌学与代谢
CiteScore
3.50
自引率
9.40%
发文量
6
审稿时长
>12 weeks
期刊介绍: Magnesium Research, the official journal of the international Society for the Development of Research on Magnesium (SDRM), has been the benchmark journal on the use of magnesium in biomedicine for more than 30 years. This quarterly publication provides regular updates on multinational and multidisciplinary research into magnesium, bringing together original experimental and clinical articles, correspondence, Letters to the Editor, comments on latest news, general features, summaries of relevant articles from other journals, and reports and statements from national and international conferences and symposiums. Indexed in the leading medical databases, Magnesium Research is an essential journal for specialists and general practitioners, for basic and clinical researchers, for practising doctors and academics.
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