Antimicrobial characterization of silver nanoparticle-coated surfaces by "touch test" method.

IF 4.9 Q2 NANOSCIENCE & NANOTECHNOLOGY
Nanotechnology, Science and Applications Pub Date : 2017-11-14 eCollection Date: 2017-01-01 DOI:10.2147/NSA.S139505
Marianne Gunell, Janne Haapanen, Kofi J Brobbey, Jarkko J Saarinen, Martti Toivakka, Jyrki M Mäkelä, Pentti Huovinen, Erkki Eerola
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引用次数: 27

Abstract

Bacterial infections, especially by antimicrobial resistant (AMR) bacteria, are an increasing problem worldwide. AMR is especially a problem with health care-associated infections due to bacteria in hospital environments being easily transferred from patient to patient and from patient to environment, and thus, solutions to prevent bacterial transmission are needed. Hand washing is an effective tool for preventing bacterial infections, but other approaches such as nanoparticle-coated surfaces are also needed. In the current study, direct and indirect liquid flame spray (LFS) method was used to produce silver nanoparticle-coated surfaces. The antimicrobial properties of these nanoparticle surfaces were evaluated with the "touch test" method against Escherichia coli and Staphylococcus aureus. It was shown in this study that in glass samples one silver nanoparticle-coating cycle can inhibit E. coli growth, whereas at least two coating cycles were needed to inhibit S. aureus growth. Silver nanoparticle-coated polyethylene (PE) and PE terephthalate samples did not inhibit bacterial growth as effectively as glass samples: three nanoparticle-coating cycles were needed to inhibit E. coli growth, and more than 30 coating cycles were needed until S. aureus growth was inhibited. To conclude, with the LFS method, it is possible to produce nanostructured large-area antibacterial surfaces which show antibacterial effect against clinically relevant pathogens. Results indicate that the use of silver nanoparticle surfaces in hospital environments could prevent health care-associated infections in vivo.

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用“触摸试验”方法研究纳米银包覆表面的抗菌特性。
细菌感染,特别是抗菌素耐药(AMR)细菌感染,是世界范围内日益严重的问题。抗菌素耐药性在医疗保健相关感染中尤其成问题,因为医院环境中的细菌很容易在患者之间以及从患者到环境之间传播,因此需要防止细菌传播的解决方案。洗手是防止细菌感染的有效工具,但也需要其他方法,如纳米颗粒涂层表面。在本研究中,采用直接和间接液体火焰喷雾(LFS)方法制备了纳米银颗粒包被表面。采用“接触试验”法对纳米颗粒表面对大肠杆菌和金黄色葡萄球菌的抗菌性能进行了评价。本研究表明,在玻璃样品中,一个银纳米颗粒包膜周期可以抑制大肠杆菌的生长,而抑制金黄色葡萄球菌的生长至少需要两个包膜周期。银纳米粒子包覆聚乙烯(PE)和对苯二甲酸聚乙烯(PE)样品对细菌生长的抑制效果不如玻璃样品:抑制大肠杆菌生长需要3个纳米粒子包覆循环,抑制金黄色葡萄球菌生长需要30多个包覆循环。综上所述,利用LFS方法,可以产生纳米结构的大面积抗菌表面,对临床相关病原体具有抗菌作用。结果表明,在医院环境中使用纳米银颗粒表面可以预防体内卫生保健相关感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Nanotechnology, Science and Applications
Nanotechnology, Science and Applications NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
11.70
自引率
0.00%
发文量
3
审稿时长
16 weeks
期刊介绍: Nanotechnology, Science and Applications is an international, peer-reviewed, Open Access journal that focuses on the science of nanotechnology in a wide range of industrial and academic applications. The journal is characterized by the rapid reporting of reviews, original research, and application studies across all sectors, including engineering, optics, bio-medicine, cosmetics, textiles, resource sustainability and science. Applied research into nano-materials, particles, nano-structures and fabrication, diagnostics and analytics, drug delivery and toxicology constitute the primary direction of the journal.
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