Effect of surface organic coatings of cellulose nanocrystals on the viability of mammalian cell lines.

IF 4.9 Q2 NANOSCIENCE & NANOTECHNOLOGY
Nanotechnology, Science and Applications Pub Date : 2017-09-28 eCollection Date: 2017-01-01 DOI:10.2147/NSA.S145891
Ambar S Jimenez, Francesca Jaramillo, Usha D Hemraz, Yaman Boluk, Karina Ckless, Rajesh Sunasee
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引用次数: 0

Abstract

Cellulose nanocrystals (CNCs) have emerged as promising candidates for a number of bio-applications. Surface modification of CNCs continues to gain significant research interest as it imparts new properties to the surface of the nanocrystals for the design of multifunctional CNCs-based materials. A small chemical surface modification can potentially lead to drastic behavioral changes of cell-material interactions thereby affecting the intended bio-application. In this work, unmodified CNCs were covalently decorated with four different organic moieties such as a diaminobutane fragment, a cyclic oligosaccharide (β-cyclodextrin), a thermoresponsive polymer (poly[N-isopropylacrylamide]), and a cationic aminomethacrylamide-based polymer using different synthetic covalent methods. The effect of surface coatings of CNCs and the respective dose-response of the above organic moieties on the cell viability were evaluated on mammalian cell cultures (J774A.1 and MFC-7), using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide and lactate dehydrogenase assays. Overall, the results indicated that cells exposed to surface-coated CNCs for 24 h did not display major changes in cell viability, membrane permeability as well as cell morphology. However, with longer exposure, all these parameters were somewhat affected, which appears not to be correlated with either anionic or cationic surface coatings of CNCs used in this study.

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纤维素纳米晶体表面有机涂层对哺乳动物细胞系活力的影响。
纤维素纳米晶体(CNCs)已成为多种生物应用的理想候选材料。对 CNCs 进行表面改性可赋予纳米晶体表面新的特性,从而设计出基于 CNCs 的多功能材料,因此一直备受研究关注。微小的化学表面改性就有可能导致细胞与材料的相互作用发生剧烈的行为变化,从而影响预期的生物应用。在这项工作中,采用不同的合成共价方法,用四种不同的有机分子(如二氨基丁烷片段、环状寡糖(β-环糊精)、热膨胀性聚合物(聚[N-异丙基丙烯酰胺])和阳离子氨基甲基丙烯酰胺基聚合物)对未修饰的 CNC 进行了共价修饰。采用 3-(4,5-二甲基噻唑-2-基)-2,5-二己基四氮唑溴化物和乳酸脱氢酶测定法,评估了数控涂层表面涂层对哺乳动物细胞培养物(J774A.1 和 MFC-7)的影响以及上述有机分子各自对细胞活力的剂量反应。总体而言,结果表明,细胞暴露于表面包覆的 CNC 24 小时后,细胞活力、膜通透性和细胞形态并未发生重大变化。然而,随着暴露时间的延长,所有这些参数都会受到一定影响,这似乎与本研究中使用的阴离子或阳离子表面涂层的 CNC 无关。
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来源期刊
Nanotechnology, Science and Applications
Nanotechnology, Science and Applications NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
11.70
自引率
0.00%
发文量
3
审稿时长
16 weeks
期刊介绍: Nanotechnology, Science and Applications is an international, peer-reviewed, Open Access journal that focuses on the science of nanotechnology in a wide range of industrial and academic applications. The journal is characterized by the rapid reporting of reviews, original research, and application studies across all sectors, including engineering, optics, bio-medicine, cosmetics, textiles, resource sustainability and science. Applied research into nano-materials, particles, nano-structures and fabrication, diagnostics and analytics, drug delivery and toxicology constitute the primary direction of the journal.
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