Nicotinic acid inhibits angiogenesis likely through cytoskeleton remodeling.

IF 1.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Organogenesis Pub Date : 2017-10-02 Epub Date: 2017-09-21 DOI:10.1080/15476278.2017.1364829

Lemen Pan, Guanfeng Yu, Xiangjian Chen, Xiaoqiang Li

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引用次数: 4

Abstract

Angiogenesis is a physiological procedure during which the new blood vessels develop from the pre-existing vessels. Uncontrolled angiogenesis is related to various diseases including cancers. Clinical inhibition of undesired angiogenesis is still under investigation. We utilized nicotinic acid, a family member of the B-vitamin niacin (vitamin B3) that has been used in the prevention and treatment of atherosclerosis or other lipid-metabolic disorders, to treat human umbilical vein endothelial cells (HUVECs) and chick chorioallantoic membrane (CAM), and investigated its influence on angiogenesis in vitro and in vivo. We found that nicotinic acid could obviously inhibit HUVEC proliferation induced by vascular endothelial growth factor. Both the in vitro and in vivo assays showed that nicotinic acid could significantly inhibit the process of angiogenesis. To further investigate the mechanism underlying the effect of nicotinic acid on angiogenesis, we found that it might function via regulating the cytoskeleton arrangements, especially the rearranging the structures of F-actin and paxillin. In summary, we discovered that nicotinic acid could obviously inhibit the process of angiogenesis by changing the angiogenesis factor expression levels and inducing the cytoskeleton rearrangement of endothelial cells.

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烟酸可能通过细胞骨架重塑抑制血管生成。

血管生成是一种生理过程，在此过程中，新血管从原有的血管发育而来。血管生成失控与包括癌症在内的多种疾病有关。临床抑制不希望的血管生成仍在研究中。我们利用已用于预防和治疗动脉粥样硬化或其他脂质代谢紊乱的b族维生素烟酸(维生素B3)家族成员烟酸治疗人脐静脉内皮细胞(HUVECs)和鸡绒毛膜尿囊膜(CAM)，并在体外和体内研究其对血管生成的影响。我们发现烟酸能明显抑制血管内皮生长因子诱导的HUVEC增殖。体外和体内实验均表明，烟酸能显著抑制血管生成过程。为了进一步探讨烟酸影响血管生成的机制，我们发现烟酸可能通过调节细胞骨架排列，特别是F-actin和paxillin的结构重排而起作用。综上所述，我们发现烟酸可以通过改变血管生成因子的表达水平，诱导内皮细胞的细胞骨架重排，从而明显抑制血管生成过程。

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来源期刊

Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY

CiteScore

4.10

自引率

4.30%

发文量

审稿时长

>12 weeks

期刊介绍： Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.