EphB receptors, mainly EphB3, contribute to the proper development of cortical thymic epithelial cells.

IF 1.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Organogenesis Pub Date : 2017-10-02 DOI:10.1080/15476278.2017.1389368

Sara Montero-Herradón, Javier García-Ceca, Agustín G Zapata

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引用次数: 8

Abstract

EphB and their ligands ephrin-B are an important family of protein tyrosine kinase receptors involved in thymocyte-thymic epithelial cell interactions known to be key for the maturation of both thymic cell components. In the present study, we have analyzed the maturation of cortical thymic epithelium in EphB-deficient thymuses evaluating the relative relevance of EphB2 and EphB3 in the process. Results support a relationship between the epithelial hypocellularity of mutant thymuses and altered development of thymocytes, lower proportions of cycling thymic epithelial cells and increased epithelial cell apoptosis. Together, these factors induce delayed development of mutant cortical TECs, defined by the expression of different cell markers, i.e. Ly51, CD205, MHCII, CD40 and β5t. Furthermore, although both EphB2 and EphB3 are necessary for cortical thymic epithelial maturation, the relevance of EphB3 is greater since EphB3-/- thymic cortex exhibits a more severe phenotype than that of EphB2-deficient thymuses.

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EphB受体，主要是EphB3，参与胸腺皮质上皮细胞的正常发育。

EphB及其配体ephrin-B是一个重要的蛋白酪氨酸激酶受体家族，参与胸腺细胞-胸腺上皮细胞的相互作用，是胸腺细胞两种成分成熟的关键。在本研究中，我们分析了ephb缺陷胸腺胸腺皮质上皮的成熟过程，评估了EphB2和EphB3在这一过程中的相对相关性。结果支持突变胸腺上皮细胞减少与胸腺细胞发育改变、循环胸腺上皮细胞比例降低和上皮细胞凋亡增加之间的关系。这些因素共同诱导突变型皮质tec的延迟发育，通过表达不同的细胞标记物，即Ly51、CD205、MHCII、CD40和β5t来定义。此外，尽管EphB2和EphB3都是胸腺皮层上皮成熟所必需的，但EphB3的相关性更大，因为EphB3-/-胸腺皮层比EphB2缺陷胸腺表现出更严重的表型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY

CiteScore

4.10

自引率

4.30%

发文量

审稿时长

>12 weeks

期刊介绍： Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.