Whole-Body Biodistribution, Dosimetry, and Metabolite Correction of [¹¹C]Palmitate: A PET Tracer for Imaging of Fatty Acid Metabolism.

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Molecular Imaging Pub Date : 2017-01-01 DOI:10.1177/1536012117734485

Nana L Christensen, Steen Jakobsen, Anna C Schacht, Ole L Munk, Aage K O Alstrup, Lars P Tolbod, Hendrik J Harms, Søren Nielsen, Lars C Gormsen

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引用次数: 20

Abstract

Introduction: Despite the decades long use of [¹¹C]palmitate positron emission tomography (PET)/computed tomography in basic metabolism studies, only personal communications regarding dosimetry and biodistribution data have been published.

Methods: Dosimetry and biodistribution studies were performed in 2 pigs and 2 healthy volunteers by whole-body [¹¹C]palmitate PET scans. Metabolite studies were performed in 40 participants (healthy and with type 2 diabetes) under basal and hyperinsulinemic conditions. Metabolites were estimated using 2 approaches and subsequently compared: Indirect [¹¹C]CO₂ release and parent [¹¹C]palmitate measured by a solid-phase extraction (SPE) method. Finally, myocardial fatty acid uptake was calculated in a patient cohort using input functions derived from individual metabolite correction compared with population-based metabolite correction.

Results: In humans, mean effective dose was 3.23 (0.02) µSv/MBq, with the liver and myocardium receiving the highest absorbed doses. Metabolite correction using only [¹¹C]CO₂ estimates underestimated the fraction of metabolites in studies lasting more than 20 minutes. Population-based metabolite correction showed excellent correlation with individual metabolite correction in the cardiac PET validation cohort.

Conclusion: First, mean effective dose of [¹¹C]palmitate is 3.23 (0.02) µSv/MBq in humans allowing multiple scans using ∼300 MBq [¹¹C]palmitate, and secondly, population-based metabolite correction compares well with individual correction.

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[11C]棕榈酸酯的全身生物分布、剂量学和代谢物校正:脂肪酸代谢成像的PET示踪剂。

导读:尽管在基础代谢研究中使用[11C]棕榈酸盐正电子发射断层扫描(PET)/计算机断层扫描已有数十年之久，但只有关于剂量学和生物分布数据的个人交流被发表。方法:采用全身[11C]棕榈酸酯PET扫描对2头猪和2名健康志愿者进行剂量学和生物分布研究。在基础和高胰岛素条件下对40名参与者(健康和2型糖尿病患者)进行代谢物研究。使用两种方法估计代谢物并随后进行比较:通过固相萃取(SPE)方法测量间接[11C]CO2释放和母体[11C]棕榈酸酯。最后，通过与基于人群的代谢物校正相比较，使用个体代谢物校正衍生的输入函数来计算患者队列中的心肌脂肪酸摄取。结果:人体平均有效剂量为3.23(0.02)µSv/MBq，肝脏和心肌吸收剂量最高。仅使用[11C]CO2估计值进行代谢物校正低估了持续时间超过20分钟的研究中代谢物的比例。在心脏PET验证队列中，基于人群的代谢物校正显示出与个体代谢物校正极好的相关性。结论:首先，[11C]棕榈酸盐在人体中的平均有效剂量为3.23(0.02)µSv/MBq，允许使用~ 300 MBq [11C]棕榈酸盐进行多次扫描;其次，基于人群的代谢物校正与个体校正相比效果更好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Imaging Biochemistry, Genetics and Molecular Biology-Biotechnology

自引率

3.60%

发文量

期刊介绍： Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.