Anti-Aging Activity of Andaliman (Zanthoxylum Acanthopodium DC) Fruit Ethanol Extract on Brain Weight and p16INK4a Expression of Hippocampus in Aging Model Rats.

Q2 Medicine

Acta Informatica Medica Pub Date : 2022-12-01 DOI:10.5455/aim.2022.30.283-286

Dwi Rita Anggraini, Syafruddin Ilyas, Poppy Anjelisa Zaitun Hasibuan, Yetty Machrina, Ambrosius Purba, Delfitri Munir, Imam Budi Putra, Betty

{"title":"Anti-Aging Activity of Andaliman (Zanthoxylum Acanthopodium DC) Fruit Ethanol Extract on Brain Weight and p16INK4a Expression of Hippocampus in Aging Model Rats.","authors":"Dwi Rita Anggraini, Syafruddin Ilyas, Poppy Anjelisa Zaitun Hasibuan, Yetty Machrina, Ambrosius Purba, Delfitri Munir, Imam Budi Putra, Betty","doi":"10.5455/aim.2022.30.283-286","DOIUrl":null,"url":null,"abstract":"Background: Physiological aging and due to oxidative stress in long term will have an impact on cellular response disorders, can caused aging of hippocampus and senility. Brain weight is known to decrease with age and p16INK4a as aging biomarkers have been investigated. Andaliman is one of typical herbal plants from North Sumatra has been widely used as an antioxidant, anti-inflammatory and anti-aging.Objective: This study was evaluated effect of andaliman (Zanthoxylum acanthopodium DC) fruit ethanol extract (AEE) on brain weight and p16INK4a expression in aging model rats.Methods: This study was carried out experimentally of 24 male wistar rats. The treatment group consisted of 4 groups; KN= negatif control (normal), KP= positif control (aging model rat), P1 and P2= aging model rat + AEE at dose 150 and 300mg/kgbw, respectively. The aging model rats were D-galactose-induced at dose of 150mg/kgbw for 8 weeks. Brain weigth were recorded by digital scales. p16INK4a expression using immunohistochemical methods. The data analysis with Anova test.Results: This study showed differences brain weight between groups (p=0.523). Brain weight in P1 (1.34±0,06) and P2 (1.30±0.09) tendency increased than KP (1.29±0.62). The p16INK4a expression between groups significant difference (p=0.041), continued with post hoc Least Significant Difference (LSD) showed p16INK4a expression in KN significant decreased than KP (p=0.027). Likewise, p16INK4a expression in P2 was significant decreased than KP (p=0.010).Conclusion: Andaliman ethanol extract at a dose 300mg/kgbw for 8 weeks was improved aging process caused D-galactose induced.","PeriodicalId":7074,"journal":{"name":"Acta Informatica Medica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f7/63/AIM-30-283.PMC9665423.pdf","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Informatica Medica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/aim.2022.30.283-286","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 1

Abstract

Background: Physiological aging and due to oxidative stress in long term will have an impact on cellular response disorders, can caused aging of hippocampus and senility. Brain weight is known to decrease with age and p16INK4a as aging biomarkers have been investigated. Andaliman is one of typical herbal plants from North Sumatra has been widely used as an antioxidant, anti-inflammatory and anti-aging.

Objective: This study was evaluated effect of andaliman (Zanthoxylum acanthopodium DC) fruit ethanol extract (AEE) on brain weight and p16INK4a expression in aging model rats.

Methods: This study was carried out experimentally of 24 male wistar rats. The treatment group consisted of 4 groups; KN= negatif control (normal), KP= positif control (aging model rat), P1 and P2= aging model rat + AEE at dose 150 and 300mg/kgbw, respectively. The aging model rats were D-galactose-induced at dose of 150mg/kgbw for 8 weeks. Brain weigth were recorded by digital scales. p16INK4a expression using immunohistochemical methods. The data analysis with Anova test.

Results: This study showed differences brain weight between groups (p=0.523). Brain weight in P1 (1.34±0,06) and P2 (1.30±0.09) tendency increased than KP (1.29±0.62). The p16INK4a expression between groups significant difference (p=0.041), continued with post hoc Least Significant Difference (LSD) showed p16INK4a expression in KN significant decreased than KP (p=0.027). Likewise, p16INK4a expression in P2 was significant decreased than KP (p=0.010).

Conclusion: Andaliman ethanol extract at a dose 300mg/kgbw for 8 weeks was improved aging process caused D-galactose induced.

Abstract Image

查看原文本刊更多论文

安达里曼(Zanthoxylum Acanthopodium DC)果乙醇提取物对衰老模型大鼠脑重及海马p16INK4a表达的抗衰老作用。

背景:生理性衰老和由于氧化应激长期会对细胞反应产生影响紊乱，可引起海马的老化和衰老。脑重量随着年龄的增长而下降，p16INK4a作为衰老的生物标志物已经被研究过。安达里曼是一种典型的北苏门答腊岛草本植物，被广泛用作抗氧化、抗炎和抗衰老。目的:研究安达里曼(Zanthoxylum acanthopodium DC)果乙醇提取物(AEE)对衰老模型大鼠脑重及p16INK4a表达的影响。方法:采用24只雄性wistar大鼠进行实验研究。治疗组分为4组;KN=阴性对照组(正常)，KP=阳性对照组(衰老模型大鼠)，P1和P2=衰老模型大鼠+ AEE，剂量分别为150和300mg/kgbw。采用150mg/kgbw剂量d -半乳糖诱导衰老模型大鼠，持续8周。脑重量用数字秤记录。p16INK4a的免疫组织化学表达。数据分析采用方差分析。结果:本研究显示各组脑重量差异(p=0.523)。脑重在P1(1.34±0.06)和P2(1.30±0.09)趋势下高于KP(1.29±0.62)。各组间p16INK4a表达差异有统计学意义(p=0.041)，继续进行事后最小显著差异(LSD)分析显示，p16INK4a表达在KN组显著低于KP组(p=0.027)。P2中p16INK4a的表达量明显低于KP (p=0.010)。结论:剂量为300mg/kgbw的安达曼乙醇提取物连续8周对d -半乳糖诱导的衰老过程有改善作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Acta Informatica Medica Medicine-Medicine (all)

CiteScore

2.90

自引率

0.00%

发文量