Lysozyme's lectin-like characteristics facilitates its immune defense function.

IF 7.2 2区生物学 Q1 BIOPHYSICS

Quarterly Reviews of Biophysics Pub Date : 2017-01-01 DOI:10.1017/S0033583517000075

Ruiyan Zhang, Lisha Wu, Thomas Eckert, Monika Burg-Roderfeld, Miguel A Rojas-Macias, Thomas Lütteke, Vadim B Krylov, Dmitry A Argunov, Aritreyee Datta, Philipp Markart, Andreas Guenther, Bengt Norden, Roland Schauer, Anirban Bhunia, Mushira Abdelaziz Enani, Martin Billeter, Axel J Scheidig, Nikolay E Nifantiev, Hans-Christian Siebert

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引用次数: 38

Abstract

Interactions between human lysozyme (HL) and the lipopolysaccharide (LPS) of Klebsiella pneumoniae O1, a causative agent of lung infection, were identified by surface plasmon resonance. To characterize the molecular mechanism of this interaction, HL binding to synthetic disaccharides and tetrasaccharides representing one and two repeating units, respectively, of the O-chain of this LPS were studied. pH-dependent structural rearrangements of HL after interaction with the disaccharide were observed through nuclear magnetic resonance. The crystal structure of the HL-tetrasaccharide complex revealed carbohydrate chain packing into the A, B, C, and D binding sites of HL, which primarily occurred through residue-specific, direct or water-mediated hydrogen bonds and hydrophobic contacts. Overall, these results support a crucial role of the Glu35/Asp53/Trp63/Asp102 residues in HL binding to the tetrasaccharide. These observations suggest an unknown glycan-guided mechanism that underlies recognition of the bacterial cell wall by lysozyme and may complement the HL immune defense function.

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溶菌酶的凝集素样特性有利于其免疫防御功能。

利用表面等离子体共振技术鉴定了人溶菌酶(HL)与肺部感染病原体肺炎克雷伯菌(Klebsiella pneumoniae O1)脂多糖(LPS)之间的相互作用。为了表征这种相互作用的分子机制，研究了HL与合成的二糖和四糖的结合，分别代表该LPS的o链的一个和两个重复单元。通过核磁共振观察了HL与双糖相互作用后的ph依赖性结构重排。HL-四糖复合物的晶体结构揭示了碳水化合物链在HL的A、B、C和D结合位点上的填充，这主要是通过残基特异性、直接或水介导的氢键和疏水接触发生的。总之，这些结果支持了Glu35/Asp53/Trp63/Asp102残基在HL与四糖结合中的关键作用。这些观察结果表明，一种未知的聚糖引导机制是溶菌酶识别细菌细胞壁的基础，并可能补充HL免疫防御功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Quarterly Reviews of Biophysics 生物-生物物理

CiteScore

12.90

自引率

1.60%

发文量

期刊介绍： Quarterly Reviews of Biophysics covers the field of experimental and computational biophysics. Experimental biophysics span across different physics-based measurements such as optical microscopy, super-resolution imaging, electron microscopy, X-ray and neutron diffraction, spectroscopy, calorimetry, thermodynamics and their integrated uses. Computational biophysics includes theory, simulations, bioinformatics and system analysis. These biophysical methodologies are used to discover the structure, function and physiology of biological systems in varying complexities from cells, organelles, membranes, protein-nucleic acid complexes, molecular machines to molecules. The majority of reviews published are invited from authors who have made significant contributions to the field, who give critical, readable and sometimes controversial accounts of recent progress and problems in their specialty. The journal has long-standing, worldwide reputation, demonstrated by its high ranking in the ISI Science Citation Index, as a forum for general and specialized communication between biophysicists working in different areas. Thematic issues are occasionally published.