Feasibility of Na18F PET/CT and MRI for Noninvasive In Vivo Quantification of Knee Pathophysiological Bone Metabolism in a Canine Model of Post-traumatic Osteoarthritis.

IF 2.2 4区 医学 Q3 BIOCHEMICAL RESEARCH METHODS
Maria I Menendez, Bianca Hettlich, Lai Wei, Michael V Knopp
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引用次数: 15

Abstract

Purpose: To assess and quantify by molecular imaging knee osseous metabolic changes serially in an in vivo canine model of posttraumatic osteoarthritis (PTOA) of the knee utilizing sodium fluoride (Na18F) positron emission tomography (PET)/computed tomography (CT) coregistered with magnetic resonance imaging (MRI).

Materials and methods: Sodium fluoride PET imaging of 5 canines was performed prior to anterior cruciate ligament transection (ACLT) and 2 times post-ACLT (3 and 12 weeks). The PET/CT was coregistered with MRI, enabling serial anatomically guided visual and quantitative three-dimensional (3D) region of interest (ROI) assessment by maximum standardized uptake value.

Results: Prior to ACLT, every 3D ROI assessed in both knees showed no Na18F uptake above background. The uptake of Na18F in the bone of the ACLT knees increased exponentially, presenting significantly higher uptake at 12 weeks in every region compared to the ACLT knees at baseline. Furthermore, the uninjured contralateral limb and the ipsilateral distal bones and joints presented Na18F uptake at 3 and 12 weeks post-ACLT.

Conclusion: This study demonstrated that Na18F PET/CT coregistered with MRI is a feasible molecular imaging biomarker to assess knee osseous metabolic changes serially in an in vivo canine model of knee PTOA. Moreover, it brings a novel musculoskeletal preclinical imaging methodology that can provide unique insights into PTOA pathophysiology.

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Na18F PET/CT和MRI在犬创伤后骨关节炎模型中无创体内量化膝关节病理生理骨代谢的可行性
目的:利用氟化钠(Na18F)正电子发射断层扫描(PET)/计算机断层扫描(CT)联合磁共振成像(MRI),对犬创伤后膝关节骨性关节炎(PTOA)体内模型的膝关节骨代谢变化进行分子成像评估和量化。材料与方法:对5只犬进行前交叉韧带横断术(ACLT)前和ACLT后(3周和12周)2次氟化钠PET显像。PET/CT与MRI共同注册,通过最大标准化摄取值进行连续解剖引导的视觉和定量三维感兴趣区域(ROI)评估。结果:在ACLT之前,双膝的每一个3D ROI评估显示没有高于背景的Na18F摄取。ACLT膝关节骨对Na18F的摄取呈指数增长,与基线时的ACLT膝关节相比,在12周时各区域的摄取均显著增加。此外,在aclt后3周和12周,未损伤的对侧肢体和同侧远端骨骼和关节出现Na18F摄取。结论:本研究表明,Na18F PET/CT与MRI共登记是一种可行的分子成像生物标志物,可连续评估犬膝关节PTOA模型的膝关节骨代谢变化。此外,它带来了一种新的肌肉骨骼临床前成像方法,可以提供独特的视角来了解pta的病理生理。
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来源期刊
Molecular Imaging
Molecular Imaging Biochemistry, Genetics and Molecular Biology-Biotechnology
自引率
3.60%
发文量
21
期刊介绍: Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.
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