Moxifloxacin Concentration and Proteomic Analysis of Aqueous Humor in Human Uveitis Associated with Oral Moxifloxacin Therapy.

IF 0.4 Q4 OPHTHALMOLOGY

Open Ophthalmology Journal Pub Date : 2017-06-12 eCollection Date: 2017-01-01 DOI:10.2174/1874364101711010107

David M Hinkle, Nicole A Kruh-Garcia, Jonathan N Kruh, Carolyn Broccardo, Priyanka Doctor, C Stephen Foster

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引用次数: 5

Abstract

Purpose: The aim was to report the aqueous humor moxifloxacin concentration and proteome profile of an individual with bilateral uveitis-like syndrome with pigment dispersion.

Methods: Multiple reactions monitoring mass spectrometry quantified the aqueous concentration of moxifloxacin in the affected individual. Shotgun proteomic analysis performed via liquid chromatography tandem mass spectrometry (LC-MS/MS) defined the protein profile in the affected individual and unaffected control samples.

Results: Moxifloxacin was present at higher than expected levels in aqueous humor 18 days following oral administration. One-third of the proteins were identified by significantly lower spectral counts in the aqueous of the individual with moxifloxacin associated uveitis compared to the unaffected control.

Conclusion: Moxifloxacin was detected in aqueous humor 18 days following the completion of oral administration. These results suggest that moxifloxacin toxicity may be responsible for the uveitis-like syndrome with pigment dispersion syndrome induced by moxifloxacin therapy.

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口服莫西沙星治疗相关葡萄膜炎患者房水中莫西沙星浓度和蛋白质组学分析。

目的:报道一例双侧葡萄膜炎样综合征伴色素分散患者房水莫西沙星浓度和蛋白质组谱。方法:多重反应监测质谱法定量测定患者体内莫西沙星水溶液浓度。通过液相色谱串联质谱(LC-MS/MS)进行的鸟枪蛋白质组学分析确定了受影响个体和未受影响的对照样品中的蛋白质谱。结果:口服莫西沙星18天后房水中莫西沙星含量高于预期。与未受影响的对照组相比，在莫西沙星相关葡萄膜炎患者的水溶液中，三分之一的蛋白质被显著降低的光谱计数所识别。结论:口服给药结束后18天房水中可检出莫西沙星。这些结果提示莫西沙星毒性可能是莫西沙星治疗引起的葡萄膜炎样综合征伴色素分散综合征的主要原因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Open Ophthalmology Journal OPHTHALMOLOGY-

CiteScore

0.50

自引率

0.00%

发文量

期刊介绍： The Open Ophthalmology Journal is an Open Access online journal, which publishes research articles, reviews/mini-reviews, letters and guest edited single topic issues in all important areas of experimental and clinical research in ophthalmology, including use of ophthalmological therapies, devices and surgical techniques. The Open Ophthalmology Journal, a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and making them freely available to researchers worldwide.