Fluorescent, Plasmonic, and Radiotherapeutic Properties of the ¹⁷⁷Lu-Dendrimer-AuNP-Folate-Bombesin Nanoprobe Located Inside Cancer Cells.

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Molecular Imaging Pub Date : 2017-01-01 DOI:10.1177/1536012117704768

Héctor Mendoza-Nava, Guillermina Ferro-Flores, Flor de María Ramírez, Blanca Ocampo-García, Clara Santos-Cuevas, Erika Azorín-Vega, Nallely Jiménez-Mancilla, Myrna Luna-Gutiérrez, Keila Isaac-Olivé

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引用次数: 37

Abstract

The integration of fluorescence and plasmonic properties into one molecule is of importance in developing multifunctional imaging and therapy nanoprobes. The aim of this research was to evaluate the fluorescent properties and the plasmonic-photothermal, therapeutic, and radiotherapeutic potential of ¹⁷⁷Lu-dendrimer conjugated to folate and bombesin with gold nanoparticles in the dendritic cavity (¹⁷⁷Lu-DenAuNP-folate-bombesin) when it is internalized in T47D breast cancer cells. The intense near-Infrared (NIR) fluorescence emitted at 825 nm from the conjugate inside cells corroborated the usefulness of DenAuNP-folate-bombesin for optical imaging. After laser irradiation, the presence of the nanosystem in cells caused a significant increase in the temperature of the medium (46.8°C, compared to 39.1°C without DenAuNP-folate-bombesin, P < 0.05), resulting in a significant decrease in cell viability (down to 16.51% ± 1.52%) due to the ¹⁷⁷Lu-DenAuNP-folate-bombesin plasmonic properties. After treatment with ¹⁷⁷Lu-DenAuNP-folate-bombesin, the T47D cell viability decreased 90% because of the radiation-absorbed dose (63.16 ± 4.20 Gy) delivered inside the cells. The ¹⁷⁷Lu-DenAuNP-folate-bombesin nanoprobe internalized in cancer cells exhibited properties suitable for optical imaging, plasmonic-photothermal therapy, and targeted radiotherapy.

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癌细胞内177lu -树突状分子- aunp -叶酸-炸弹素纳米探针的荧光、等离子体和放射治疗特性

将荧光和等离子体特性整合到一个分子中对于开发多功能成像和治疗纳米探针具有重要意义。本研究的目的是评估177lu -树突状大分子(177Lu-DenAuNP-folate-bombesin)在T47D乳腺癌细胞内化时的荧光特性、等离子光热、治疗和放射治疗潜力。177lu -树突状大分子与叶酸和树蛋白结合，并在树突状腔中与金纳米颗粒结合。细胞内共轭物在825 nm处发出的强近红外(NIR)荧光证实了DenAuNP-folate-bombesin在光学成像中的作用。激光照射细胞后，纳米系统的存在导致培养基温度显著升高(46.8°C，与不含DenAuNP-folate-bombesin的39.1°C相比，P < 0.05)，由于177Lu-DenAuNP-folate-bombesin的等离子体性质，导致细胞活力显著下降(降至16.51%±1.52%)。经177Lu-DenAuNP-folate-bombesin处理后，细胞内的辐射吸收剂量(63.16±4.20 Gy)使T47D细胞活力下降90%。癌细胞内化的177Lu-DenAuNP-folate-bombesin纳米探针显示出适合光学成像、等离子体光热治疗和靶向放疗的特性。

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来源期刊

Molecular Imaging Biochemistry, Genetics and Molecular Biology-Biotechnology

自引率

3.60%

发文量

期刊介绍： Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.