Preclinical Multimodal Molecular Imaging Using 18F-FDG PET/CT and MRI in a Phase I Study of a Knee Osteoarthritis in In Vivo Canine Model.

IF 2.2 4区 医学 Q3 BIOCHEMICAL RESEARCH METHODS
Maria I Menendez, Bianca Hettlich, Lai Wei, Michael V Knopp
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引用次数: 13

Abstract

The aim of this study was to use a multimodal molecular imaging approach to serially assess regional metabolic changes in the knee in an in vivo anterior cruciate ligament transection (ACLT) canine model of osteoarthritis (OA). Five canine underwent ACLT in one knee and the contralateral knee served as uninjured control. Prior, 3, 6, and 12 weeks post-ACLT, the dogs underwent 18F-fluoro-d-glucose (18F-FDG) positron emission tomography (PET)/computed tomography (CT) and magnetic resonance imaging (MRI). The MRI was coregistered with the PET/CT, and 3-dimensional regions of interest (ROIs) were traced manually and maximum standardized uptake values (SUVmax) were evaluated. 18F-fluoro-d-glucose SUVmax in the ACLT knee ROIs was significantly higher compared to the uninjured contralateral knees at 3, 6, and 12 weeks. Higher 18F-FDG uptake observed in ACLT knees compared to the uninjured knees reflects greater metabolic changes in the injured knees over time. Knee 18F-FDG uptake in an in vivo ACLT canine model using combined PET/CT and MRI demonstrated to be highly sensitive in the detection of metabolic alterations in osseous and nonosteochondral structures comprising the knee joint. 18F-fluoro-d-glucose appeared to be a capable potential imaging biomarker for early human knee OA diagnosis, prognosis, and management.

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使用18F-FDG PET/CT和MRI进行临床前多模态分子成像在犬体内膝关节骨关节炎模型中的I期研究。
本研究的目的是使用多模态分子成像方法来连续评估骨关节炎(OA)犬体内前交叉韧带横断(ACLT)模型膝关节的区域代谢变化。5只犬单侧膝关节行ACLT,对侧膝关节作为未损伤对照。在aclt之前、3、6和12周后,狗接受了18f -氟-葡萄糖(18F-FDG)正电子发射断层扫描(PET)/计算机断层扫描(CT)和磁共振成像(MRI)。MRI与PET/CT共同登记,手动追踪三维感兴趣区域(roi),并评估最大标准化摄取值(SUVmax)。在第3周、第6周和第12周,ACLT膝关节ROIs的18f -氟-d-葡萄糖SUVmax明显高于未受伤的对侧膝关节。与未受伤的膝盖相比,在ACLT膝盖中观察到更高的18F-FDG摄取反映了受伤膝盖随时间的更大代谢变化。在活体ACLT犬模型中,使用PET/CT和MRI联合检测膝关节18F-FDG摄取在检测膝关节骨和非骨软骨结构代谢改变方面具有高度敏感性。18f -氟-d-葡萄糖似乎是早期人类膝关节OA诊断、预后和治疗的潜在成像生物标志物。
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来源期刊
Molecular Imaging
Molecular Imaging Biochemistry, Genetics and Molecular Biology-Biotechnology
自引率
3.60%
发文量
21
期刊介绍: Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.
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