The neuronal differentiation microenvironment is essential for spinal cord injury repair.

IF 1.6 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Organogenesis Pub Date : 2017-07-03 Epub Date: 2017-06-09 DOI:10.1080/15476278.2017.1329789
Yannan Zhao, Zhifeng Xiao, Bing Chen, Jianwu Dai
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引用次数: 33

Abstract

Spinal cord injury (SCI) often leads to substantial disability due to loss of motor function and sensation below the lesion. Neural stem cells (NSCs) are a promising strategy for SCI repair. However, NSCs rarely differentiate into neurons; they mostly differentiate into astrocytes because of the adverse microenvironment present after SCI. We have shown that myelin-associated inhibitors (MAIs) inhibited neuronal differentiation of NSCs. Given that MAIs activate epidermal growth factor receptor (EGFR) signaling, we used a collagen scaffold-tethered anti-EGFR antibody to attenuate the inhibitory effects of MAIs and create a neuronal differentiation microenvironment for SCI repair. The collagen scaffold modified with anti-EGFR antibody prevented the inhibition of NSC neuronal differentiation by myelin. After transplantation into completely transected SCI animals, the scaffold-linked antibodies induced production of nascent neurons from endogenous and transplanted NSCs, which rebuilt the neuronal relay by forming connections with each other or host neurons to transmit electrophysiological signals and promote functional recovery. Thus, a scaffold-based strategy for rebuilding the neuronal differentiation microenvironment could be useful for SCI repair.

神经元分化微环境对脊髓损伤修复至关重要。
脊髓损伤(SCI)通常由于损伤部位以下的运动功能和感觉丧失而导致严重的残疾。神经干细胞(NSCs)是一种很有前途的脊髓损伤修复策略。然而,NSCs很少分化为神经元;由于脊髓损伤后存在不利的微环境,它们大多分化为星形胶质细胞。我们已经证明髓磷脂相关抑制剂(MAIs)抑制NSCs的神经元分化。考虑到MAIs激活表皮生长因子受体(EGFR)信号,我们使用胶原支架连接的抗EGFR抗体来减弱MAIs的抑制作用,并为脊髓损伤修复创造神经元分化微环境。抗egfr抗体修饰的胶原支架可阻止髓磷脂对NSC神经元分化的抑制。将支架连接抗体移植到完全横切的SCI动物体内后,诱导内源性和移植的NSCs产生新生神经元,这些新生神经元通过相互或与宿主神经元形成连接来重建神经元中继,传递电生理信号,促进功能恢复。因此,基于支架的重建神经元分化微环境的策略可能对脊髓损伤修复有用。
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来源期刊
Organogenesis
Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY
CiteScore
4.10
自引率
4.30%
发文量
6
审稿时长
>12 weeks
期刊介绍: Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.
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