Genomics Perspectives on Metabolism, Survival Strategies, and Biotechnological Applications of Brettanomyces bruxellensis LAMAP2480.

IF 1.2 Q2 Biochemistry, Genetics and Molecular Biology

Journal of Molecular Microbiology and Biotechnology Pub Date : 2017-01-01 Epub Date: 2017-06-09 DOI:10.1159/000471924

Liliana Godoy, Evelyn Silva-Moreno, Wladimir Mardones, Darwin Guzman, Francisco A Cubillos, Angélica Ganga

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引用次数: 9

Abstract

Wine production is an important commercial issue for the liquor industry. The global production was estimated at 275.7 million hectoliters in 2015. The loss of wine production due to Brettanomyces bruxellensis contamination is currently a problem. This yeast causes a "horse sweat" flavor in wine, which is an undesired organoleptic attribute. To date, 6 B. bruxellensis annotated genome sequences are available (LAMAP2480, AWRI1499, AWRI1608, AWRI1613, ST05.12/22, and CBS2499), and whole genome comparisons between strains are limited. In this article, we reassembled and reannotated the genome of B. bruxellensis LAMAP2480, obtaining a 27-Mb assembly with 5.5 kb of N50. In addition, the genome of B. bruxellensis LAMAP2480 was analyzed in the context of spoilage yeast and potential as a biotechnological tool. In addition, we carried out an exploratory transcriptomic analysis of this strain grown in synthetic wine. Several genes related to stress tolerance, micronutrient acquisition, ethanol production, and lignocellulose assimilation were found. In conclusion, the analysis of the genome of B. bruxellensis LAMAP2480 reaffirms the biotechnological potential of this strain. This research represents an interesting platform for the study of the spoilage yeast B. bruxellensis.

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bruxellbrettanomyces LAMAP2480代谢、生存策略及生物技术应用的基因组学视角

葡萄酒生产是白酒行业的一个重要商业问题。2015年全球产量估计为2.757亿升。由于布鲁塞尔酿酒酵母污染造成的葡萄酒生产损失是目前的一个问题。这种酵母在葡萄酒中产生“马汗”味，这是一种不希望的感官属性。迄今为止，已有6条布鲁塞尔芽孢杆菌带注释的基因组序列(LAMAP2480、AWRI1499、AWRI1608、AWRI1613、ST05.12/22和CBS2499)，菌株之间的全基因组比较有限。本文对bruxellensis LAMAP2480的基因组进行了重组和重新注释，获得了一个27 mb的片段，其中N50为5.5 kb。此外，在腐败酵母的背景下，分析了布鲁塞尔芽孢杆菌LAMAP2480的基因组及其作为生物技术工具的潜力。此外，我们还对该菌株在合成酒中生长进行了探索性转录组学分析。发现了几个与胁迫耐受性、微量营养素获取、乙醇生产和木质纤维素同化有关的基因。综上所述，bruxellensis LAMAP2480的基因组分析再次证实了该菌株的生物技术潜力。本研究为腐殖酵母的研究提供了一个有趣的平台。

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来源期刊

Journal of Molecular Microbiology and Biotechnology 生物-生物工程与应用微生物

CiteScore

3.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： We are entering a new and exciting era of microbiological study and application. Recent advances in the now established disciplines of genomics, proteomics and bioinformatics, together with extensive cooperation between academic and industrial concerns have brought about an integration of basic and applied microbiology as never before.