Relative Strengths and Regulation of Different Promoter-Associated Sequences for Various blaSHV Genes and Their Relationships to β-Lactam Resistance.

IF 1.2 Q2 Biochemistry, Genetics and Molecular Biology

Journal of Molecular Microbiology and Biotechnology Pub Date : 2017-01-01 Epub Date: 2017-03-23 DOI:10.1159/000458708

Yao Zhai, Zhao Zhang, Zhanwei Wang, Yusheng Chen, Qi Wang, Yuan Lv, Jingping Yang, Tong Zhao, Yatao Guo, Zhancheng Gao

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引用次数: 4

Abstract

Aims: This work investigated the relative strengths of different blaSHV promoter-associated sequences and their regulation function in blaSHV expression and β-lactam resistance.

Methods: Recombinant plasmids with the promoter-associated sequences (P-W, P-S, P-IS, and P-WPD), tac promoter, and combined fragments of promoter and blaSHV were separately constructed and transformed into Escherichia coli DH5α. The relative strengths of the promoters indicated by the intensities of green fluorescent protein and the mRNA expression levels of blaSHV were compared. The minimum inhibitory concentration and extended spectrum β-lactamase phenotypes were evaluated.

Results: The relative strengths were ranked as P-tac > P-WPD > P-IS > P-S > P-W. The mRNA expression and β-lactam resistance levels of the different promoter-associated sequence groups were generally consistent with the strength rank, but the extent of gfp and blaSHV mRNA levels varied significantly in each group. The β-lactam resistance levels were inconsistent with the strength rank in certain blaSHV groups. In relation to the different promoter-associated sequences, blaSHV-ESBLs displayed significantly different change modes of β-lactam resistance compared with blaSHV-non-ESBLs.

Conclusion: The mRNA expression and β-lactam resistance of the blaSHV showed consistencies and inconsistencies with the strengths of the promoter-associated sequences. The mechanisms accounting for these discrepancies need further investigation.

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不同blaSHV基因启动子相关序列的相对优势、调控及其与β-内酰胺抗性的关系

目的:研究不同blaSHV启动子相关序列的相对优势及其在blaSHV表达和β-内酰胺抗性中的调控作用。方法:分别构建含有启动子相关序列(P-W、P-S、P-IS、P-WPD)、tac启动子、启动子与blaSHV组合片段的重组质粒，转染大肠杆菌DH5α。比较绿色荧光蛋白强度指示的启动子的相对强弱和blaSHV mRNA的表达水平。对最小抑制浓度和扩展谱β-内酰胺酶表型进行了评价。结果:P-tac > P-WPD > P-IS > P-S > P-W。不同启动子相关序列组的mRNA表达量和β-内酰胺抗性水平与强度等级基本一致，但各组gfp和blaSHV mRNA水平差异程度显著。某些blaSHV组β-内酰胺抗性水平与强度等级不一致。针对不同启动子相关序列，blaSHV-ESBLs与blaSHV-non-ESBLs的β-内酰胺抗性变化模式存在显著差异。结论:blaSHV的mRNA表达和β-内酰胺抗性与启动子相关序列的强度存在一致性和不一致性。造成这些差异的机制需要进一步调查。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Molecular Microbiology and Biotechnology 生物-生物工程与应用微生物

CiteScore

3.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： We are entering a new and exciting era of microbiological study and application. Recent advances in the now established disciplines of genomics, proteomics and bioinformatics, together with extensive cooperation between academic and industrial concerns have brought about an integration of basic and applied microbiology as never before.