Genetic Polymorphisms of TLR4 and MICA are Associated with Severity of Trachoma Disease in Tanzania.

Autoimmune and Infectious Diseases: Open Access Pub Date : 2016-06-01 Epub Date: 2016-06-03 DOI:10.16966/2470-1025.116

Muneer Abbas, Noureddine Berka, Mozna Khraiwesh, Ali Ramadan, Victor Apprey, Paulette Furbert-Harris, Thomas Quinn, Hassan Brim, Georgia Dunston

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引用次数: 3

Abstract

Aim: To examine the association of TLR4 Asp299Gly and MICA exon 5 microsatellites polymorphisms with severity of trachoma in a sub-Saharan East Africa population of Tanzanian villagers.

Methods: The samples were genotyped for MICA exon 5 microsatellites and the TLR4 299 A/G polymorphism by Restriction Fragment Length Polymorphism (RFLP), and GeneScan^®, respectively. The association of TLR4 Asp299Gly and MICA exon 5 microsatellites with inflammatory trachoma (TI) and trichiasis (TI) were examined.

Results: The results showed an association between TLR4 and MICA polymorphisms and trachoma disease severity, as well as with protection. TLR4 an allele was significantly associated with inflammatory trachoma (p=0.0410), while the G allele (p=0.0410) was associated with protection.

Conclusion: TLR4 and MICA may modulate the risk of severity to trachoma disease by modulating the immune response to Ct. In addition; the increased frequency of MICA-A9 heterozygote in controls may suggest a positive selection of these alleles in adaptation to environments where Ct is endemic.

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TLR4和MICA基因多态性与坦桑尼亚沙眼病的严重程度相关

目的:研究TLR4 Asp299Gly和MICA外显子5微卫星多态性与撒哈拉以南东非坦桑尼亚村民沙眼严重程度的关系。方法:采用限制性片段长度多态性(RFLP)和GeneScan®分别对MICA外显子5微卫星和TLR4 299 A/G多态性进行基因分型。研究了TLR4 Asp299Gly和MICA外显子5微卫星与炎症性沙眼(TI)和倒睫(TI)的相关性。结果:结果显示TLR4和MICA多态性与沙眼疾病严重程度以及保护作用之间存在关联。tlr4a等位基因与炎性沙眼显著相关(p=0.0410)，而G等位基因与保护相关(p=0.0410)。结论:TLR4和MICA可能通过调节对Ct的免疫反应来调节沙眼疾病严重程度的风险。除了;对照组中MICA-A9杂合子频率的增加可能表明，在适应Ct流行的环境时，这些等位基因出现了正选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Autoimmune and Infectious Diseases: Open Access

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