Vitamin C promotes the proliferation of human adipose-derived stem cells via p53-p21 pathway.

IF 1.6 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Organogenesis Pub Date : 2016-07-02 Epub Date: 2016-05-26 DOI:10.1080/15476278.2016.1194148
Peihua Zhang, Jin Li, Yawei Qi, Yaqing Zou, Li Liu, Xudong Tang, Jianfeng Duan, Hongwei Liu, Guofang Zeng
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引用次数: 27

Abstract

Although adipose-derived stem cells (ADSCs) have demonstrated a promising potential for the applications of cell-based therapy and regenerative medicine, excessive reactive oxygen species (ROS) are harmful to ADSCs cell survival and proliferation. Vitamin C is an important antioxidant, and is often added into culture media as an essential micronutrient. However, its roles on the proliferation of human ADSCs have not been studied. Therefore, in this study, human ADSCs were isolated, and detected by flow cytometry for the analysis of their cell surface antigens. Cell proliferation and cell cycle progression were measured with cell counting kit-8 assay and flow cytometry, respectively. Western blotting was used to detect the expression levels of cyclin E1, p53, p21, and CDK2 proteins. The effect of vitamin C pretreatment on the production of hydrogen peroxide (H2O2)-mediated ROS in the ADSCs was evaluated by flow cytometry. Our results indicated that vitamin C treatment significantly increased cell proliferation, and changed the cell cycle distribution of ADSCs by decreasing the percentage of G1 phase, and concurrently increased the percentage of S and G2/M phase. Western blot analysis indicated that vitamin C treatment up-regulated the expression levels of cyclin E1 and CDK2, but down-regulated p53 and p21 proteins expression, which contributed to cell proliferation and cell cycle progression. Vitamin C pretreatment significantly reduced the production of H2O2-induced ROS in the ADSCs. These findings suggest that vitamin C can promote the proliferation and cell cycle progression in the ADSCs possibly through regulation of p53-p21 signal pathway.

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维生素C通过p53-p21途径促进人脂肪来源干细胞的增殖。
尽管脂肪源性干细胞(ADSCs)在细胞治疗和再生医学中具有广阔的应用前景,但过多的活性氧(ROS)对ADSCs的存活和增殖是有害的。维生素C是一种重要的抗氧化剂,通常作为一种必需微量营养素添加到培养基中。然而,其在人ADSCs增殖中的作用尚未被研究。因此,本研究分离了人ADSCs,用流式细胞术检测其细胞表面抗原。分别用细胞计数试剂盒-8法和流式细胞术检测细胞增殖和细胞周期进展。Western blotting检测细胞周期蛋白E1、p53、p21、CDK2蛋白的表达水平。采用流式细胞术观察维生素C预处理对ADSCs过氧化氢(H2O2)介导的ROS生成的影响。我们的研究结果表明,维生素C处理显著增加了ADSCs的增殖,并通过降低G1期的百分比改变了细胞周期分布,同时增加了S期和G2/M期的百分比。Western blot分析表明,维生素C处理可上调细胞周期蛋白E1和CDK2的表达水平,下调p53和p21蛋白的表达,促进细胞增殖和细胞周期的进展。维生素C预处理显著降低h2o2诱导的ADSCs中ROS的产生。提示维生素C可能通过调控p53-p21信号通路促进ADSCs的增殖和细胞周期进程。
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来源期刊
Organogenesis
Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY
CiteScore
4.10
自引率
4.30%
发文量
6
审稿时长
>12 weeks
期刊介绍: Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.
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