MiR-10b decreases sensitivity of glioblastoma cells to radiation by targeting AKT.

IF 1.9 3区生物学 Q2 BIOLOGY

Journal of Biological Research-Thessaloniki Pub Date : 2016-06-24 eCollection Date: 2016-12-01 DOI:10.1186/s40709-016-0051-x

Limin Zhen, Jian Li, Mingran Zhang, Kun Yang

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引用次数: 2

Abstract

Background: Glioblastomas are the most aggressive brain tumors with extremely poor prognosis despite advances in treatment techniques. MiR-10b is highly expressed in glioblastoma and regulates cell proliferation, migration and invasion. Here, we examined the role of MiR-10b on radiotherapy of glioblastomas.

Methods: MiR-10b mimic or anti-MiR-10b inhibitor was transfected in glioblastoma cells. WST-1 assay was used to examine the effect of MiR-10b on proliferation of transfected glioblastoma cells after radiation treatment. Apoptosis was examined by caspase 3/7 activity and TUNEL assay. The western blot was used to evaluate protein expression.

Results: Altered expression of MiR-10b changed the radiation-induced inhibitory effect on proliferation of glioblastoma cells with dose-dependent manner. MiR-10b decreased radiation-induced apoptosis in glioblastoma cells by activation of caspase 3/7 and inhibition Bcl-2 expression. MiR-10b enhances migration and invasion of glioblastoma cells in presence of radiation. In addition, MiR-10b decreased the sensitivity of glioblastoma cells to radiotherapy by activation of p-AKT expression.

Conclusions: MiR-10b might be a potential biomarker to predict radiotherapy response and prognosis in glioblastomas.

Abstract Image

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MiR-10b通过靶向AKT降低胶质母细胞瘤细胞对辐射的敏感性。

背景:胶质母细胞瘤是最具侵袭性的脑肿瘤，尽管治疗技术有所进步，但预后极差。MiR-10b在胶质母细胞瘤中高表达，调控细胞增殖、迁移和侵袭。在这里，我们研究了MiR-10b在胶质母细胞瘤放疗中的作用。方法:在胶质母细胞瘤细胞中转染MiR-10b模拟物或抗MiR-10b抑制剂。采用WST-1法检测MiR-10b对放射治疗后转染的胶质母细胞瘤细胞增殖的影响。用caspase 3/7活性和TUNEL法检测细胞凋亡。western blot检测蛋白表达。结果:MiR-10b表达的改变改变了辐射对胶质母细胞瘤细胞增殖的抑制作用，并呈剂量依赖性。MiR-10b通过激活caspase 3/7和抑制Bcl-2表达，减少辐射诱导的胶质母细胞瘤细胞凋亡。MiR-10b在辐射存在下增强胶质母细胞瘤细胞的迁移和侵袭。此外，MiR-10b通过激活p-AKT表达降低胶质母细胞瘤细胞对放疗的敏感性。结论:MiR-10b可能是预测胶质母细胞瘤放疗反应和预后的潜在生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biological Research-Thessaloniki 生物-生物学

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Biological Research-Thessaloniki is a peer-reviewed, open access, international journal that publishes articles providing novel insights into the major fields of biology. Topics covered in Journal of Biological Research-Thessaloniki include, but are not limited to: molecular biology, cytology, genetics, evolutionary biology, morphology, development and differentiation, taxonomy, bioinformatics, physiology, marine biology, behaviour, ecology and conservation.