The Vibrio cholerae ToxR Regulon Encodes Host-Specific Chemotaxis Proteins that Function in Intestinal Colonization.

SOJ microbiology & infectious diseases Pub Date : 2015-01-01 Epub Date: 2015-12-03 DOI:10.15226/sojmid/3/3/00141

Pradeep Selvaraj, Rohit Gupta, Kenneth M Peterson

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引用次数: 8

Abstract

Virulence gene regulation in Vibrio cholerae is under the control of the ToxR-ToxT regulatory cascade. Chemotaxis and net motility have been shown to influence the infectivity of Vibrio cholerae. V. cholerae toxR mutants do not synthesize proteins required for chemotaxis towards mucus. The inability of the toxR mutant strain to recognize and swim towards mucus is due to their failure to synthesize AcfB, a methyl-accepting chemotaxis protein. AcfB has previously been shown to be involved in intestinal colonization using the infant mouse model of cholera infection. Wild type V. cholerae recognizes galactose-6-sulfate in the capillary tube assay whereas V. cholerae acfB mutants fail to migrate into the capillary tubes. Vibrio strains carrying a mutation in tcpI, a ToxR regulated gene found within the Vibrio Pathogenicity Island (VPI), which encodes a methyl accepting chemotaxis protein are fully chemotactic towards mucus and galactose-6-sulfate.

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霍乱弧菌ToxR调控子编码在肠道定植中起作用的宿主特异性趋化蛋白。

霍乱弧菌的毒力基因调控受ToxR-ToxT调控级联的控制。趋化性和净运动性已被证明影响霍乱弧菌的传染性。霍乱弧菌toxR突变体不能合成对黏液趋化所需的蛋白质。toxR突变株无法识别并向粘液游去是由于它们无法合成AcfB，一种接受甲基的趋化蛋白。在霍乱感染的幼鼠模型中，AcfB先前已被证明参与肠道定植。野生型霍乱弧菌在毛细管实验中识别半乳糖-6-硫酸酯，而霍乱弧菌acfB突变体不能迁移到毛细管中。携带tcpI突变的弧菌菌株对粘液和半乳糖-6-硫酸完全趋化，tcpI是弧菌致病性岛(VPI)中发现的ToxR调控基因，其编码甲基接受趋化蛋白。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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SOJ microbiology & infectious diseases

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