The fatty acid chain elongase, Elovl1, is required for kidney and swim bladder development during zebrafish embryogenesis.

IF 1.6 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Organogenesis Pub Date : 2016-04-02 Epub Date: 2016-04-14 DOI:10.1080/15476278.2016.1172164
Sushil Bhandari, Joon No Lee, Young-Il Kim, In-Koo Nam, Su-Jung Kim, Se-Jin Kim, SeongAe Kwak, Gi-Su Oh, Hyung-Jin Kim, Hyun Ju Yoo, Hong-Seob So, Seong-Kyu Choe, Raekil Park
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引用次数: 26

Abstract

Very long chain fatty acids are required for sphingolipid synthesis, lipid homeostasis, myelin formation, epidermal permeability, and retinal function. Seven different enzymes are known to be involved in the elongation cycle of fatty acids, with different chain-length specificities. Elovl1 is one of those enzymes whose function has been linked mainly to the synthesis of sphingolipids and the epidermal barrier. However, the role of Elovl1 in organogenesis is not clear. In zebrafish, 2 Elovl1 genes, elovl1a and elovl1b, are highly expressed in the swim bladder, and elovl1b is also expressed in the kidney. We found that both elovl1 knockdown embryos contain increased levels of long chain fatty acids from carbon number 14 to 20 as compared to control embryos. Oil-Red-O staining shows that yolk lipid consumption is greatly reduced, whereas lipid droplets accumulate within the swim bladder. Notably, knockdown of either elovl1a or elovl1b affects the expression of genes involved in swim bladder development and impairs inflation of the swim bladder. Consistent with its expression in the pronephros, knockdown of elovl1b alone affects the expression of genes required for kidney development and reduces renal clearance. Our findings strongly suggest that both elovl1 genes are a key determinant of swim bladder and kidney development in zebrafish, which may be comparatively applicable to lung and kidney development in humans.

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在斑马鱼胚胎发育过程中,脂肪酸链延长酶Elovl1是肾脏和鱼鳔发育所必需的。
长链脂肪酸是鞘脂合成、脂质稳态、髓磷脂形成、表皮通透性和视网膜功能所必需的。已知有七种不同的酶参与脂肪酸的延伸周期,具有不同的链长特异性。Elovl1是其中一种酶,其功能主要与鞘脂的合成和表皮屏障有关。然而,Elovl1在器官发生中的作用尚不清楚。在斑马鱼中,2个Elovl1基因,elovl1a和elovl1b在鱼鳔中高表达,elovl1b也在肾脏中表达。我们发现,与对照胚胎相比,两个elovl1敲低的胚胎含有从碳数14到20的长链脂肪酸水平增加。油-红- o染色显示蛋黄脂质消耗大大减少,而脂滴在鱼鳔内积聚。值得注意的是,elovl1a或elovl1b的敲低会影响与鱼鳔发育有关的基因的表达,并损害鱼鳔的膨胀。与其在肾原中的表达一致,单独敲低elovl1b会影响肾脏发育所需基因的表达,并降低肾脏清除率。我们的研究结果强烈表明,两个elovl1基因都是斑马鱼鱼鳔和肾脏发育的关键决定因素,这可能相对适用于人类肺和肾脏的发育。
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来源期刊
Organogenesis
Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY
CiteScore
4.10
自引率
4.30%
发文量
6
审稿时长
>12 weeks
期刊介绍: Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.
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