Application of next-generation sequencing to investigation of norovirus diversity in shellfish collected from two coastal sites in Japan from 2013 to 2014.

IF 0.4 4区农林科学 Q4 VETERINARY SCIENCES

Japanese Journal of Veterinary Research Pub Date : 2016-05-01

Saiki Imamura, Mika Haruna, Tomoko Goshima, Hiromi Kanezashi, Tsukasa Okada, Keiko Akimoto

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引用次数: 0

Abstract

A better understanding of the role played by shellfish regarding the manner of pathogen contamination, persistence, and selection may help considering epidemiology of noroviruses. Thus, norovirus genotype profiles in shellfish (Crassostrea gigas and Mitilus galloprovincialis) were investigated by using Next-generation sequencing (NGS) technology. In genogroup I (GI), 7 genotypes (abbreviated as GI.2 to GI.7, and GI.9) were detected from C. gigas, whereas 9 genotypes (GI.1 to GI.9) were detected from M. galloprovincialis. The genotype with the highest proportion found in both C. gigas and M. galloprovincialis was GI.4, and the second highest was GI.3. In genogroup II (GII), 17 genotypes (GII.1 to GII.9, GII.11 to GII.17, GII.21 and GI.22) were detected from C. gigas, whereas 16 genotypes (GII.1 to GII.8, GII.11 to GII.17, GII.21 and GI.22) were detected from M. galloprovincialis. The genotype with the highest proportion in both C. gigas and M. galloprovincialis was GII.4, the next highest differed between C. gigas and M. galloprovincialis. To our knowledge, this study may be the first trial to utilize the latest technology in this field, and reveal the diversity of norovirus genotypes present in shellfish.

本刊更多论文

应用新一代测序技术调查2013 - 2014年日本两处沿海贝类诺瓦克病毒多样性。

更好地了解贝类在病原体污染方式、持久性和选择方面所起的作用可能有助于考虑诺如病毒的流行病学。因此，利用新一代测序(NGS)技术对贝类(长牡蛎和加洛贝类)的诺如病毒基因型进行了研究。在基因组I (GI)中，从gigas中检测到7个基因型(缩写为GI.2 ~ GI.7和GI.9)，从M. galloprovincialis中检测到9个基因型(缩写为GI.1 ~ GI.9)。gigas和galloprovincialis中比例最高的基因型为GI.4，其次为GI.3。在基因组II (GII)中，从gigas中检测到GII.1 ~ GII.9、GII.11 ~ GII.17、GII.21和GII. 22 17种基因型，从galloprovincialis中检测到GII.1 ~ GII.8、GII.11 ~ GII.17、GII.21和GII. 22 16种基因型。gigas和M. galloprovincialis中所占比例最高的基因型为gi1 .4, gigas和M. galloprovincialis之间的差异次之。据我们所知，这项研究可能是首次利用该领域的最新技术，揭示了贝类中诺如病毒基因型的多样性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Japanese Journal of Veterinary Research 农林科学-兽医学

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： The Japanese Journal of Veterinary Research (JJVR) quarterly publishes peer-reviewed articles on all aspects of veterinary science. JJVR was originally published as a “University Journal” of veterinary science at Hokkaido University from more than 60 years ago. Currently, JJVR, is Japan’s leading scientific veterinary journal, and provides valuable information for the development of veterinary science by welcoming contributions from researchers worldwide. JJVR offers online submission for Regular Papers, Short Communications, and Review Articles that are unpublished and not being considered for publication elsewhere. Research areas include: Anatomy, Physiology, Biochemistry, Pharmacology, Microbiology, Infectious diseases, Parasitology, Laboratory Animal Science and Medicine, Internal Medicine, Surgery, Pathology, Theriogenology, Molecular Medicine, Public Health, Radiation Biology, Toxicology, Wildlife Biology and Medicine, Veterinary Hygiene, The other fields related to veterinary science.