Heterogeneous phase fibrinolysis rates by damped oscillation rheometry.

IF 1 4区医学 Q4 BIOPHYSICS

Biorheology Pub Date : 2016-07-29 DOI:10.3233/BIR-15089

Jae-Suk Lee, Makoto Kaibara, Edgar A O'Rear

{"title":"Heterogeneous phase fibrinolysis rates by damped oscillation rheometry.","authors":"Jae-Suk Lee, Makoto Kaibara, Edgar A O'Rear","doi":"10.3233/BIR-15089","DOIUrl":null,"url":null,"abstract":"Background: Devices gauging viscoelastic properties of blood during coagulation like the thromboelastograph support fundamental research as well as point of care needs. Associated fibrinolysis data are based on endogenous species or plasminogen activator added to a homogeneous sample prior to clot formation. Digestion in a monolithic structure differs from the physical situation of thrombolytic therapy where surface reactions dominate.Objective: This study aims to develop rheological testing for heterogeneous phase fibrinolysis.Method: Fibrinolysis rates were determined by phase change of a solid clot induced by autologous plasma/streptokinase (SK) in a rheometer sensitive to viscous damping.Results: Initial slope or overall change in the logarithmic damping factor indicated fibrinolytic rates. Rates depended on clot geometry, phase volumes, clot composition and SK concentration.Conclusion: The damped oscillation rheometer can be adapted to determine relative rates of heterogeneous fibrinolysis in vitro.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"53 2","pages":"81-92"},"PeriodicalIF":1.0000,"publicationDate":"2016-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-15089","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biorheology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.3233/BIR-15089","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOPHYSICS","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Devices gauging viscoelastic properties of blood during coagulation like the thromboelastograph support fundamental research as well as point of care needs. Associated fibrinolysis data are based on endogenous species or plasminogen activator added to a homogeneous sample prior to clot formation. Digestion in a monolithic structure differs from the physical situation of thrombolytic therapy where surface reactions dominate.

Objective: This study aims to develop rheological testing for heterogeneous phase fibrinolysis.

Method: Fibrinolysis rates were determined by phase change of a solid clot induced by autologous plasma/streptokinase (SK) in a rheometer sensitive to viscous damping.

Results: Initial slope or overall change in the logarithmic damping factor indicated fibrinolytic rates. Rates depended on clot geometry, phase volumes, clot composition and SK concentration.

Conclusion: The damped oscillation rheometer can be adapted to determine relative rates of heterogeneous fibrinolysis in vitro.

查看原文本刊更多论文

阻尼振荡流变法测定非均相纤维蛋白溶解率。

背景:测量凝血过程中血液粘弹性特性的设备，如血栓弹性仪，支持基础研究和护理点需求。相关的纤溶数据是基于内源性物种或纤溶酶原激活剂在血栓形成之前添加到均匀样品中。整体结构的消化不同于表面反应占主导地位的溶栓治疗的物理情况。目的:建立非均相纤溶的流变学检测方法。方法:在黏性阻尼敏感流变仪中，采用自体血浆/链激酶(SK)诱导的固体凝块的相变法测定纤维蛋白溶解率。结果:对数阻尼因子的初始斜率或总体变化指示纤溶率。速率取决于凝块几何形状、相体积、凝块组成和SK浓度。结论:阻尼振荡流变仪可用于体外测定非均质纤溶的相对速率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biorheology 医学-工程：生物医学

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biorheology is an international interdisciplinary journal that publishes research on the deformation and flow properties of biological systems or materials. It is the aim of the editors and publishers of Biorheology to bring together contributions from those working in various fields of biorheological research from all over the world. A diverse editorial board with broad international representation provides guidance and expertise in wide-ranging applications of rheological methods to biological systems and materials. The scope of papers solicited by Biorheology extends to systems at different levels of organization that have never been studied before, or, if studied previously, have either never been analyzed in terms of their rheological properties or have not been studied from the point of view of the rheological matching between their structural and functional properties. This biorheological approach applies in particular to molecular studies where changes of physical properties and conformation are investigated without reference to how the process actually takes place, how the forces generated are matched to the properties of the structures and environment concerned, proper time scales, or what structures or strength of structures are required.